PXD020900

PXD020900 is an original dataset announced via ProteomeXchange.

Title	Streamlining of defenses against copper makes host-restricted pathogen reliant on custom-made operon
Description	Copper is both essential and toxic to living beings, which therefore tightly control its intracellular concentration. At the host-pathogen interface, copper is used by phagocytic cells to kill invading microorganisms. We investigated copper homeostasis in the whooping cough agent Bordetella pertussis, which lives in the human respiratory mucosa and has no environmental reservoir. B. pertussis has considerably streamlined copper homeostasis mechanisms relative to other Gram-negative bacteria. Its single remaining defense line against copper intoxication consists in a metallochaperone diverted for copper passivation and two enzymes involved in peroxide detoxification, which together fight two stresses encountered in phagolysosomes. The three proteins are encoded by an original, composite operon assembled in an environmental ancestor and which is under sensitive control by copper. Interestingly, this system appears to play a role in persistent infection in the nasal cavity of B. pertussis-infected mice. Combining responses to co-occurring stresses in a tailored operon reveals a new strategy adopted by a host-restricted pathogen to optimize survival at minimal energy expenditure.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_04:23:52.539.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020900
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SALIOU Jean-Michel
SpeciesList	scientific name: Bordetella bronchiseptica; NCBI TaxID: 518;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-13 22:40:31	ID requested
⏵ 1	2021-09-09 04:23:53	announced

submitter keyword: Bordetella pertussis, Copper homeostasis, Phagocytosis, oxidative stress, Evolution, Metallochaperone, host-restricted pathogen

Saliou Jean-Michel
contact affiliation	Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US41 – UMS 2014 – PLBS, F-59000 Lille, France
contact email	Jean-michel.saliou@pasteur-lille.fr
lab head
SALIOU Jean-Michel
contact affiliation	Institut Pasteur de Lille
contact email	jean-michel.saliou@pasteur-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020900

PRIDE project URI

• PRIDE
  1. PXD020900
     1. Label: PRIDE project
     2. Name: Streamlining of defenses against copper makes host-restricted pathogen reliant on custom-made operon