PXD020899

PXD020899 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis of proteomic and phosphoproteomic of granulosa cells during follicular atresia in porcine
Description	Ovarian follicular atresia is a natural physiological process, but its mechanism is not fully understood. In this study, a quantitative proteomic and phosphoproteomic of granulosa cell (GC) in the healthy (H), slightly atretic (SA), and atretic follicles (A) of porcine were performed by TMT labeling, enrichment of phosphopeptides and LC-MS/MS analysis. Altogether, 6,201 proteins were quantified, and 4,723 phosphorylation sites of 1,760 proteins were quantified. There are 24 (11 up, 13 down) and 50 (29 up, 21 down) proteins with FC>5 in H/SA and H/A, respectively. In addition, there are 20 (H/SA, up) and 39 (H/A, up) phosphosites with FC>7, which could serve as potential biomarkers to distinguish different quality categories of follicles. The results of western blotting and immunofluorescence indicated the reliability of the proteomic analysis. Further analysis of the differential expressed proteins (DEPs) and phosphorylated proteins (DEPPs) revealed some key proteins (e.g. MIF, beta catenin, integrin β2), key phosphosites (e.g. S76 of Caspase6, S22 and S636 of Lamin A/C), pathways (e.g. apoptosis, regulation of actin cytoskeleton pathway), transcription factors (e.g. STAT5A, FOXO1, BCLAF1), and kinases (e.g. PBK, CDK5, CDK12, AKT3) that involved in atresia process. Our proteomic and phosphoproteomic profiling and functional research comprehensively analyze the dynamic changes of protein expression and phosphorylation during follicular atresia, and gave a new explanation for the regulation of this process.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:18:08.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feng Feng
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-13 22:30:06	ID requested
⏵ 1	2021-09-09 02:18:08	announced

Yang F, Liu Q, Chen Y, Ye H, Wang H, Zeng S, Integrative Proteomic and Phosphoproteomic Analyses of Granulosa Cells During Follicular Atresia in Porcine. Front Cell Dev Biol, 8():624985(2020) [pubmed]

submitter keyword: Porcine

follicular atresia

granulosa cell

proteomic

phosphoproteomic

Shenming Zeng
contact affiliation	College of Animal Science and Technology, China Agricultural University.College of Animal Science and Technology, China Agricultural University.
contact email	zengsm@cau.edu.cn
lab head
Feng Feng
contact affiliation	College of Animal Science and Technology, China Agricultural University
contact email	chinafy6868@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020899
     1. Label: PRIDE project
     2. Name: Integrative analysis of proteomic and phosphoproteomic of granulosa cells during follicular atresia in porcine