PXD020887

PXD020887 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Grafting induced pluripotent stem cells-derived tenocytes contribute to recovery of motor function after Achilles tendon rupture in rat
Description	Self-renewal of tendons is rare since the vascular formation inside is extremely poor, thus reconstructive surgery using autologous tendons has been often taken place in the case of severe injury. However, the rate of re-injury after surgery is relatively high, and collection of autologous tendons leads muscle weakness which results in prolonged rehabilitation. Here, we introduce the induced pluripotent stem cells (iPSCs)-based technology aiming at developing a new therapeutic option after tendon injury. Firstly, we derived tenocytes from human iPSCs by recapitulating the normal progression of step-wise narrowing fate decisions in the vertebrate embryo. We used single-cell RNA sequencing to analyze the developmental trajectory of iPSCs-derived tenocytes. We then demonstrated that grafting iPSCs-tenocytes contributed to recovery of motor function after Achilles tendon injury in rat via engraftments and paracrine effects. The biomechanical strength of regenerated tendons recovered comparable to that of healthy tendons. We propose that the iPSCs-tenocytes will provide a novel therapeutic option in tendon injury.
HostingRepository	jPOST
AnnounceDate	2021-07-01
AnnouncementXML	Submission_2022-09-18_02:26:21.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Iwasaki Mio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; deamidated L-asparagine; deamidated L-glutamine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); alpha-amino acetylated residue; Applied Biosystems iTRAQ(TM) multiplexed quantitation chemistry
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-13 00:46:28	ID requested
1	2021-06-30 08:00:08	announced
2	2021-09-06 01:34:22	announced	2021-09-06: Updated PubMed.
⏵ 3	2022-09-18 02:26:22	announced	2022-09-18: Updated FTP location.

Publication List

Nakajima T, Nakahata A, Yamada N, Yoshizawa K, Kato TM, Iwasaki M, Zhao C, Kuroki H, Ikeya M, Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture. Nat Commun, 12(1):5012(2021) [pubmed]

Nakajima T, Nakahata A, Yamada N, Yoshizawa K, Kato TM, Iwasaki M, Zhao C, Kuroki H, Ikeya M, Grafting of iPS cell-derived tenocytes promotes motor function recovery after Achilles tendon rupture. Nat Commun, 12(1):5012(2021) [pubmed]

Keyword List

submitter keyword: iPSC, Tendon, Tenocyte

submitter keyword: iPSC, Tendon, Tenocyte

Contact List

Mio Iwasaki
lab head
Iwasaki Mio
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000939/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000939/