PXD020875

PXD020875 is an original dataset announced via ProteomeXchange.

Title	An improved protocol to purify bacterial pili from Lactobacillus rhamnosus GG based on multimodal chromatography
Description	Pili are polymeric proteins located at the cell surface of bacteria. These filamentous proteins are essential molecular actors playing pivotal role in bacterial adhesion with the surrounding environment. These proteins are found both in Gram-negative and positive bacteria but differ in structural organization. Purifying these high molecular weight proteins is challenging and certainly has slow down their characterization. Here, we proposed a chromatography-based protocol, mainly based on multimodal chromatography, to purify sortase-dependant SpaCBA pili from Lactobacillus rhamnosus GG (LGG). LGG is a Gram-positive bacterium that display probiotic properties notably through its SpaCBA pili. This protocol does not require specific antibodies nor complex laboratory equipment since all chromatography steps can be performed simply with a syringe, including the multimodal chromatography step. This simple protocol could be useful to numerous laboratories to purify easily pili from LGG. Therefore, the present work should boost specific studies dedicated to LGG SpaCBA pili and the characterization of the interactions occurring with their protein partners at the molecular level. Moreover, this straightforward purification process might be extended to the purification of sortase-dependant pili from other gram-positive bacteria.
HostingRepository	PRIDE
AnnounceDate	2020-12-03
AnnouncementXML	Submission_2021-01-06_06:27:25.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020875
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Raphael Dos Santos Morais
SpeciesList	scientific name: Lactobacillus rhamnosus GG; NCBI TaxID: 568703;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	orbitrap; autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-12 08:51:21	ID requested
1	2020-12-02 23:12:43	announced
⏵ 2	2021-01-06 06:27:26	announced	2021-01-06: Updated publication reference for PubMed record(s): 33391233.

Dos Santos Morais R, El-Kirat-Chatel S, Burgain J, Simard B, Barrau S, Paris C, Borges F, Gaiani C, GG Based on Multimodal Chromatography. Front Microbiol, 11():609880(2020) [pubmed]

submitter keyword: Lactobacillus rhamnosus GG, SpaCBA pili, purification, multimodal chromatography, light scattering, AFM

Claire GAIANI
contact affiliation	Pr. Claire GAIANI - Directrice Adjointe du LIBio Membre de l'Institut Universitaire de France Université de Lorraine LIBio – Laboratoire d’Ingénierie des Biomolécules 2 avenue de la Forêt de Haye - BP 20163 54505 Vandoeuvre-lès-Nancy - FRANCE
contact email	claire.gaiani@univ-lorraine.fr
lab head
Raphael Dos Santos Morais
contact affiliation	Université de Lorraine, LIBio, F-54000, Nancy, France
contact email	raphael.dos-santos-morais@univ-lorraine.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020875
     1. Label: PRIDE project
     2. Name: An improved protocol to purify bacterial pili from Lactobacillus rhamnosus GG based on multimodal chromatography