PXD020869

PXD020869 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Stationary Growth Stage and Nutritional Deficiency Adaptation Reveals Brucella Abortus Virulence Mechanisms
Description	Brucellosis, an important bacterial zoonosis caused by Brucella species, has drawn increased attention around the world. As an intracellular pathogen, the ability of Brucella to deal with stress within the host cell is closely related to its virulence. The survival pressure on Brucella within a phagosome is considered similar to that during the stationary phase. Here, label-free proteomics approach was used to study the adaptive response of Brucella abortus (B. abortus) in the stationary stage. 182 down-regulated and 140 up-regulated proteins were found in the stationary-phase B. abortus. B. abortus adapted to adverse environmental changes by regulating virulence, reproduction, transcription, translation, stress response, and energy production. In addition, both logarithmic and stationary-phase B. abortus were treated with short-term starvation. The logarithmic-phase B. abortus restricted cell reproduction and energy utilization in response to nutritional stress. Additionally, the expression levels of some virulence-related proteins were identified as being significantly regulated during the transition from logarithmic to stationary phase or under starvation treatment, such as Type IV secretion system protein (T4SS), VjbR, and integration host factor (IHF). Altogether, we outlined adaptive mechanisms that B. abortus could employ during the growth and compared the differences between logarithmic and stationary-phase B. abortus in response to starvation.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:41:04.697.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianghua Yang
SpeciesList	scientific name: Brucella abortus str. 2308 A; NCBI TaxID: 641140;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-12 04:51:13	ID requested
⏵ 1	2021-09-09 00:41:05	announced

Yang J, Liu M, Liu J, Liu B, He C, Chen Z, . Front Microbiol, 11():598797(2020) [pubmed]

submitter keyword: B. abortus

proteomics

logarithmic phase

stationary phase

nutritional stress

zeliang Chen
contact affiliation	Shenyang Agricultural University
contact email	zeliangchen@yahoo.com
lab head
Jianghua Yang
contact affiliation	Shenyang Agricultural University
contact email	Yjh20191207@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020869

PRIDE project URI

• PRIDE
  1. PXD020869
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Stationary Growth Stage and Nutritional Deficiency Adaptation Reveals Brucella Abortus Virulence Mechanisms