PXD020868

PXD020868 is an original dataset announced via ProteomeXchange.

Title	Nocardamine-dependent iron uptake in Pseudomonas aeruginosa : exclusive implication of the FoxA outer membrane transporter
Description	Iron is a key nutrient for almost all living organisms and paradoxically poorly soluble and consequently poorly bioavailable. To get access to this metal, bacteria have developed many different strategies. One of the most common consists of the use of siderophores, small compounds chelating ferric iron with a very high affinity. Many bacteria are able to produce their own siderophores or use those produced by other microorganisms (exosiderophores) in a piracy strategy. Pseudomonas aeruginosa produces two siderophores, pyoverdine and pyochelin and is able to use a large panel of exosiderophores. We investigated the ability of P. aeruginosa to use nocardamine (NOCA) and ferrioxamine B (DFOB) as exosiderophores under iron-limited planktonic growth conditions. Proteomic and RT-qPCR approaches showed an induction of the transcription and expression of the outer membrane transporter FoxA in the presence of NOCA or DFO in the bacterial environment. Expression of the proteins of the heme or pyoverdine and pyochelin dependent iron uptake pathways were not affected in the presence of these two tris-hydroxamate siderophores. 55Fe uptake assays using foxA mutants demonstrated that ferri-NOCA was exclusively transported by FoxA, while ferri-DFO was transported by FoxA and at least one another unidentified transporter. The crystal structure of FoxA in complex with NOCA revealed very similar siderophore binding sites between NOCA and DFO. Iron uptake by hydroxamate exosiderophores in P. aeruginosa cells is discussed in the light of these results.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-29_08:22:07.655.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020868
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-12 01:36:19	ID requested
⏵ 1	2021-04-29 08:22:08	announced

Dataset with its publication pending

submitter keyword: Pseudomonas, iron uptake, FoxA, nocardamine

Isabelle Schalk
contact affiliation	CNRS, UMR7242, UMR7242, Université de Strasbourg, ESBS, Bld Sébastien Brant, F-67412 Illkirch, Strasbourg, France
contact email	isabelle.schalk@unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD020868

PRIDE project URI

• PRIDE
  1. PXD020868
     1. Label: PRIDE project
     2. Name: Nocardamine-dependent iron uptake in Pseudomonas aeruginosa : exclusive implication of the FoxA outer membrane transporter