PXD020824

PXD020824 is an original dataset announced via ProteomeXchange.

Title	Analysis of Amyloid Plaque Composition in two mouse models of Alzheimer’s disease
Description	MALDI mass spectrometry imaging (MSI) enables label-free, spatially resolved analysis of a wide range of analytes in tissue sections. Quantitative analysis of MSI datasets is typically performed on single pixels or manually assigned regions of interest (ROI). However, many sparse, small objects such as Alzheimer’s disease (AD) brain deposits of amyloid peptides called plaques are neither single pixels nor ROI. Here, we propose a new approach to facilitate comparative computational evaluation of amyloid plaque-like objects by MSI: a fast PLAQUE PICKER tool that enables statistical evaluation of heterogeneous amyloid peptide composition. Comparing two AD mouse models, APP NL-G-F and APP PS1, we identified distinct heterogeneous plaque populations in the NL-G-F model, but only one class of plaques in the PS1 model. We propose quantitative metrics for the comparison of technical and biological MSI replicates.
HostingRepository	PRIDE
AnnounceDate	2020-10-15
AnnouncementXML	Submission_2020-11-04_23:22:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Enzlein
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-10 09:04:10	ID requested
1	2020-10-14 23:23:05	announced
2	2020-10-15 01:02:00	announced	2020-10-15: Updated project metadata.
⏵ 3	2020-11-04 23:22:22	announced	2020-10-15: Updated project metadata. 2020-11-05: Updated publication reference for PubMed record(s): 33138378.

Enzlein T, Cordes J, Munteanu B, Michno W, Serneels L, De Strooper B, Hanrieder J, Wolf I, Ch, á, vez-Guti, é, rrez L, Hopf C, Computational Analysis of Alzheimer Amyloid Plaque Composition in 2D- and Elastically Reconstructed 3D-MALDI MS Images. Anal Chem, 92(21):14484-14493(2020) [pubmed]

submitter keyword: MALDI, MS imaging, Linear positive mode, Alzheimer, Abeta, Amyloid plaques

Carsten Hopf
contact affiliation	Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.
contact email	c.hopf@hs-mannheim.de
lab head
Thomas Enzlein
contact affiliation	Center for Mass Spectrometry and Optical Spectroscopy (CeMOS), Mannheim University of Applied Sciences, Paul-Wittsack Str. 10, 68163, Mannheim, Germany.
contact email	t.enzlein@hs-mannheim.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD020824

PRIDE project URI

• PRIDE
  1. PXD020824
     1. Label: PRIDE project
     2. Name: Analysis of Amyloid Plaque Composition in two mouse models of Alzheimer’s disease