PXD020822

PXD020822 is an original dataset announced via ProteomeXchange.

Title	CPEB1 or CPEB1 (T171A, S177A) interacting proteome analysis on C2C12 cells
Description	CPEB1 regulates cellular function by post-transcriptionally controlling its targeted transcripts' translation. After bound to CPEs, CPEB1 recruits cytoplasmic poly (A) polymerase GLD2 to elongate the poly (A) tail for the maintenance of mRNA stability. The stability of mRNA is positively correlated with translational output. It is unexamined whether CPEB1 interacts with translation machinery to regulation translation. Previous reports demonstrate that phosphorylation is required for its regulation on translation. However, it is not well understood whether phosphorylation is essential for CPEB1 interaction with translation machinery or not. Here, we performed mass spectrometry on C2 cells transfected with CPEB1-mVenus or CPEB1 (T171A, S177A)-mVenus after IgG or mVenus antibody immunoprecipitation. After analysis, we identify CPEB1 interacting proteins and the phosphorylation dependent interacting proteins such as ribosomal proteins. Thus, our data suggest that CPEB1 regulate translation by interacting with translation machinery in a phosphorylation dependent manner.
HostingRepository	PRIDE
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2021-11-04_00:32:29.284.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020822
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tom Cheung
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-10 06:31:11	ID requested
⏵ 1	2021-11-04 00:32:29	announced

Dataset with its publication pending

submitter keyword: C2C12, timsTOF, antibody immunoprecipitation

Tom H. Cheung
contact affiliation	Hong Kong University of Science and Technology
contact email	tcheung@ust.hk
lab head
Tom Cheung
contact affiliation	Hong Kong University of Science and Technology
contact email	tcheung@ust.hk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020822
     1. Label: PRIDE project
     2. Name: CPEB1 or CPEB1 (T171A, S177A) interacting proteome analysis on C2C12 cells