PXD020812

PXD020812 is an original dataset announced via ProteomeXchange.

Title	Adipose MDM2 regulates systemic insulin sensitivity through nuclear import of MORC2 and the PPARγ cofactor LIPIN1
Description	The intimate association between obesity and type II diabetes urges for a deeper understanding of adipocyte function. We and others have previously delineated a role for the tumor suppressor p53 in adipocyte biology. Here, we show that mice haploinsufficient for MDM2, a key regulator of p53, in their adipose stores suffer from overt obesity, insulin resistance, and hepatic steatosis. These mice had decreased levels of circulating palmitoleic acid (non-esterified fatty acid (NEFA) 16:1) concomitant with impaired visceral adipose tissue expression of Scd1 and Ffar4. A similar decrease in Scd and Ffar4 expression was found in in vitro differentiated adipocytes with perturbed MDM2 expression. Mechanistically, lowered MDM2 levels led to nuclear exclusion of the transcriptional cofactors, MORC2 and LIPIN1, thus hampering adipocyte function by antagonizing LIPIN1-mediated PPARγ coactivation. Collectively, these data argue for a p53-independent role of MDM2 in controlling adipocyte function through LIPIN1.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_13:58:57.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Puglia
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-09 23:15:01	ID requested
⏵ 1	2022-02-15 13:58:58	announced

Hallenborg P, Jensen BAH, Fj, æ, re E, Petersen RK, Belma, â, ti MS, Rasmussen SS, Gunnarsson JP, Lauritzen P, Cheng KKY, Hermansson M, Sonne SB, Ejsing CS, Xu A, Kratchmarova I, Kr, ü, ger M, Madsen L, Kristiansen K, Blagoev B, Adipose MDM2 regulates systemic insulin sensitivity. Sci Rep, 11(1):21839(2021) [pubmed]

submitter keyword: MDM2, obesity, LIPIN1, MORC2, C/EBP, PPARγ

Blagoy Blagoev
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense M, Denmark
contact email	bab@bmb.sdu.dk
lab head
Michele Puglia
contact affiliation	University of Southern Denmark
contact email	michelep@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD020812

PRIDE project URI

• PRIDE
  1. PXD020812
     1. Label: PRIDE project
     2. Name: Adipose MDM2 regulates systemic insulin sensitivity through nuclear import of MORC2 and the PPARγ cofactor LIPIN1