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PXD020797

PXD020797 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLongitudinal Protein Profiling using Targeted Proteomics and Recombinant Standards
DescriptionSpike-in of standards of known concentrations used in proteomics-based workflows is an attractive approach for both accurate and precise multiplexed protein quantification. Here, a quantitative method based on targeted proteomics analysis of plasma proteins using isotope-labelled recombinant standards originating from the Human Protein Atlas project has been established. The standards were individually quantified prior to being employed in the final multiplex assay. The assays are mainly directed towards actively secreted proteins produced in the liver, but may also originate from other parts of the human body. This study included 21 proteins classified by FDA as either drug targets or approved clinical protein biomarkers. We describe the use of this multiplex assay for profiling a well-defined human cohort with sample collection spanning over a one-year period. Samples were collected at four different time points which allowed for a longitudinal analysis to assess the variable plasma proteome within individuals. Two assays towards APOA1 and APOB had available clinical data and the two assays were benchmarked against each other. The clinical assay is based on antibodies and shows high correlation between the two orthogonal methods suggesting that targeted proteomics with highly parallel, multiplex analysis is an attractive alternative to antibody-based protein assays.
HostingRepositoryPanoramaPublic
AnnounceDate2021-03-15
AnnouncementXMLSubmission_2021-03-15_07:23:37.063.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFredrik Edfors
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-07 14:13:49ID requested
12021-03-15 07:23:37announced
Publication List
Kotol D, Hunt H, Hober A, Karlsson MJ, Forsström B, Gummesson A, Bergström G, Fagerberg L, Uhlén M, Edfors F, Longitudinal Plasma Protein Profiling Using Targeted Proteomics and Recombinant Protein Standards. J Proteome Res, 19(12):4815-4825(2020) [pubmed]
Keyword List
submitter keyword: PRM PrEST Plasma SCAPIS
Contact List
Andreas Hober
contact affiliationScience For Life Laboratory
contact emailandreas.hober@scilifelab.se
lab head
Fredrik Edfors
contact affiliationScience For Life Laboraotry
contact emailfredrik.edfors@scilifelab.se
dataset submitter
Full Dataset Link List
Panorama Public dataset URI