PXD020797

PXD020797 is an original dataset announced via ProteomeXchange.

Title	Longitudinal Protein Profiling using Targeted Proteomics and Recombinant Standards
Description	Spike-in of standards of known concentrations used in proteomics-based workflows is an attractive approach for both accurate and precise multiplexed protein quantification. Here, a quantitative method based on targeted proteomics analysis of plasma proteins using isotope-labelled recombinant standards originating from the Human Protein Atlas project has been established. The standards were individually quantified prior to being employed in the final multiplex assay. The assays are mainly directed towards actively secreted proteins produced in the liver, but may also originate from other parts of the human body. This study included 21 proteins classified by FDA as either drug targets or approved clinical protein biomarkers. We describe the use of this multiplex assay for profiling a well-defined human cohort with sample collection spanning over a one-year period. Samples were collected at four different time points which allowed for a longitudinal analysis to assess the variable plasma proteome within individuals. Two assays towards APOA1 and APOB had available clinical data and the two assays were benchmarked against each other. The clinical assay is based on antibodies and shows high correlation between the two orthogonal methods suggesting that targeted proteomics with highly parallel, multiplex analysis is an attractive alternative to antibody-based protein assays.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-15
AnnouncementXML	Submission_2021-03-15_07:23:37.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Fredrik Edfors
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-07 14:13:49	ID requested
⏵ 1	2021-03-15 07:23:37	announced

Kotol D, Hunt H, Hober A, Karlsson MJ, Forsstr, ö, m B, Gummesson A, Bergstr, ö, m G, Fagerberg L, Uhl, é, n M, Edfors F, Longitudinal Plasma Protein Profiling Using Targeted Proteomics and Recombinant Protein Standards. J Proteome Res, 19(12):4815-4825(2020) [pubmed]

submitter keyword: PRM PrEST Plasma SCAPIS

Andreas Hober
contact affiliation	Science For Life Laboratory
contact email	andreas.hober@scilifelab.se
lab head
Fredrik Edfors
contact affiliation	Science For Life Laboraotry
contact email	fredrik.edfors@scilifelab.se
dataset submitter

Panorama Public dataset URI