PXD020792 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Trans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting SERCA2b Ca2+ pump |
Description | The endoplasmic reticulum (ER) contains various enzymes that metabolize fatty acids (FAs). How FA metabolic enzymes cooperate with other ER functions, such as calcium (Ca2+) accumulation and its regulated release to the cytosol, is elusive. Trans-2-enoyl-CoA reductase (TER) is a FA reductase present in the ER membrane, and catalyzes the last step of FA elongation cycle and sphingosine degradation pathway. Here we show that TER directly binds to an ER Ca2+ pump, sarco(endo)plasmic reticulum Ca2+-ATPase 2b (SERCA2b), and regulates its activity. Thapsigargin, a specific SERCA inhibitor, inhibits this binding. TER binds to SERCA2b through its conserved C-terminal region. TER overexpression suppresses SERCA2b ATPase activity in microsomal membranes. Depletion of TER increases the ER Ca2+ storage and accelerates SERCA2b-dependent Ca2+ uptake to the ER after ligand-induced Ca2+ release. These results demonstrate that TER is a negative regulator of SERCA2b, implying the direct linkage of FA metabolism and Ca2+ accumulation in the ER. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-13 |
AnnouncementXML | Submission_2021-01-13_01:25:26.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Toshiaki Sakisaka |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LCMS-IT-TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-07 04:20:02 | ID requested | |
⏵ 1 | 2021-01-13 01:25:26 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: fatty acid metabolism, endoplasmic reticulum, calcium, calcium ATPase, calcium transport, protein-protein interaction |
Contact List
Toshiaki Sakisaka |
contact affiliation | Division of Membrane Dynamics, Department of Physiology and Cell Biology, Kobe University School of Medicine |
contact email | sakisaka@med.kobe-u.ac.jp |
lab head | |
Toshiaki Sakisaka |
contact affiliation | Kobe University Graduate School of Medicine |
contact email | sakisaka@med.kobe-u.ac.jp |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020792
- Label: PRIDE project
- Name: Trans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting SERCA2b Ca2+ pump