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PXD020792 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTrans-2-enoyl-CoA reductase limits Ca2+ accumulation in the endoplasmic reticulum by inhibiting SERCA2b Ca2+ pump
DescriptionThe endoplasmic reticulum (ER) contains various enzymes that metabolize fatty acids (FAs). How FA metabolic enzymes cooperate with other ER functions, such as calcium (Ca2+) accumulation and its regulated release to the cytosol, is elusive. Trans-2-enoyl-CoA reductase (TER) is a FA reductase present in the ER membrane, and catalyzes the last step of FA elongation cycle and sphingosine degradation pathway. Here we show that TER directly binds to an ER Ca2+ pump, sarco(endo)plasmic reticulum Ca2+-ATPase 2b (SERCA2b), and regulates its activity. Thapsigargin, a specific SERCA inhibitor, inhibits this binding. TER binds to SERCA2b through its conserved C-terminal region. TER overexpression suppresses SERCA2b ATPase activity in microsomal membranes. Depletion of TER increases the ER Ca2+ storage and accelerates SERCA2b-dependent Ca2+ uptake to the ER after ligand-induced Ca2+ release. These results demonstrate that TER is a negative regulator of SERCA2b, implying the direct linkage of FA metabolism and Ca2+ accumulation in the ER.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterToshiaki Sakisaka
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-07 04:20:02ID requested
12021-01-13 01:25:26announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: fatty acid metabolism, endoplasmic reticulum, calcium, calcium ATPase, calcium transport, protein-protein interaction
Contact List
Toshiaki Sakisaka
contact affiliationDivision of Membrane Dynamics, Department of Physiology and Cell Biology, Kobe University School of Medicine
contact emailsakisaka@med.kobe-u.ac.jp
lab head
Toshiaki Sakisaka
contact affiliationKobe University Graduate School of Medicine
contact emailsakisaka@med.kobe-u.ac.jp
dataset submitter
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