PXD020777-2
PXD020777 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons |
Description | At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2020-10-27 |
AnnouncementXML | Submission_2020-10-27_18:08:02.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Tim Veth |
SpeciesList | scientific name: Rattus norvegicus; NCBI TaxID: 10116; |
ModificationList | Phospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4) |
Instrument | TSQ Altis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-08-06 12:03:21 | ID requested | |
1 | 2020-08-14 16:32:24 | announced | |
⏵ 2 | 2020-10-27 18:08:03 | announced | 2020-10-27: Added publication |
Publication List
van Gelder CAGH, Penning R, Veth TS, Catsburg LAE, Hoogenraad CC, MacGillavry HD, Altelaar M, Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons. Mol Cell Proteomics, 19(12):1952-1968(2020) [pubmed] |
Keyword List
submitter keyword: mGluR-LTD, proteomics, phosphoproteomics, synaptic plasticity, protein translation, AMPA receptor, bioorthogonal amino acid tagging, metabolic labeling, tandem mass tags |
Contact List
AFM Altelaar | |
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contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht |
contact email | m.altelaar@uu.nl |
lab head | |
Tim Veth | |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht |
contact email | t.s.veth@uu.nl |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |