PXD020777

PXD020777 is an original dataset announced via ProteomeXchange.

Title	Temporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons
Description	At neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses.
HostingRepository	PanoramaPublic
AnnounceDate	2020-10-27
AnnouncementXML	Submission_2020-10-27_18:08:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Tim Veth
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	Phospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-06 12:03:21	ID requested
1	2020-08-14 16:32:24	announced
⏵ 2	2020-10-27 18:08:03	announced	2020-10-27: Added publication

van Gelder CAGH, Penning R, Veth TS, Catsburg LAE, Hoogenraad CC, MacGillavry HD, Altelaar M, Temporal Quantitative Proteomics of mGluR-induced Protein Translation and Phosphorylation in Neurons. Mol Cell Proteomics, 19(12):1952-1968(2020) [pubmed]

submitter keyword: mGluR-LTD, proteomics, phosphoproteomics, synaptic plasticity, protein translation, AMPA receptor, bioorthogonal amino acid tagging, metabolic labeling, tandem mass tags

AFM Altelaar
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht
contact email	m.altelaar@uu.nl
lab head
Tim Veth
contact affiliation	Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht
contact email	t.s.veth@uu.nl
dataset submitter

Panorama Public dataset URI