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PXD020777

PXD020777 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTemporal quantitative proteomics of mGluR-induced protein translation and phosphorylation in neurons
DescriptionAt neuronal synapses, activation of group I metabotropic glutamate receptors (mGluR1/5) triggers a form of long-term depression (mGluR-LTD) that relies on new protein synthesis and the internalization of AMPA-type glutamate receptors. Dysregulation of these processes has been implicated in the development of mental disorders such as autism spectrum disorders and therefore merit a better understanding on a molecular level. Here, to study mGluR-induced signaling pathways, we integrated quantitative phosphoproteomics with the analyses of newly synthesized proteins via bio-orthogonal amino acids (azidohomoalanine) in a pulsed labeling strategy in cultured hippocampal neurons stimulated with DHPG, a specific agonist for group I mGluRs. We identified several kinases with important roles in DHPG-induced mGluR activation, which we confirmed using small molecule kinase inhibitors. Furthermore, changes in the AMPA receptor endocytosis pathway in both protein synthesis and protein phosphorylation were identified, whereby Intersectin-1 was validated as a novel player in this pathway. This study revealed several new insights into the molecular pathways downstream of group I mGluR activation in hippocampal neurons, and provides a rich resource for further analyses.
HostingRepositoryPanoramaPublic
AnnounceDate2020-08-14
AnnouncementXMLSubmission_2020-08-14_16:32:24.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTim Veth
SpeciesList scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationListPhospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-06 12:03:21ID requested
12020-08-14 16:32:24announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mGluR-LTD, proteomics, phosphoproteomics, synaptic plasticity, protein translation, AMPA receptor, bioorthogonal amino acid tagging, metabolic labeling, tandem mass tags
Contact List
AFM Altelaar
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht
contact emailm.altelaar@uu.nl
lab head
Tim Veth
contact affiliationBiomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences & Netherlands Proteomics Center, 3584 CH Utrecht
contact emailt.s.veth@uu.nl
dataset submitter
Full Dataset Link List
Panorama Public dataset URI