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PXD020774

PXD020774 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTranscriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems
DescriptionPrescribed exercise has the potential to ameliorate tissue repair or regeneration and therefore is gaining increasing importance in regenerative rehabilitation. Osteocytes are an appealing type of bone cell to target by regenerative rehabilitation protocols due to their mechanosensitive and secretory nature. Making use of tandem liquid chromatography-mass spectrometry (LC-MSMS) as well as curated bioinformatics, we examined the secretome of mouse and human osteocytic bone cells cultured under cyclic tension delivered by a computer-controlled bioreactor. Quantitative SWATH MS analysis of the secretome secreted by mechanically stimulated cells revealed differential expression of 13 out of 759 secreted factors in mouse and 16 out of 276 in human osteocytic cells. GO enrichment analysis suggested that response to oxidative stress was the most predominant biological process in mouse and cholesterol or lipoprotein-related in human cells. Ossification and bone remodelling and manganese transport were 2 overrepresented process networks common to both mouse and human osteocytic cells
HostingRepositoryPRIDE
AnnounceDate2021-06-08
AnnouncementXMLSubmission_2021-06-07_21:36:38.845.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDavid Boocock
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-06 04:43:59ID requested
12021-06-07 21:36:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mouse osteocyte, human osteocyte, SWATH, secretome, tissue regeneration, mechanically stimulated
Contact List
David John Boocock
contact affiliationJohn van Geest Cancer Research Centre, Nottingham Trent University
contact emaildavid.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliationNottingham Trent University
contact emaildavid.boocock@ntu.ac.uk
dataset submitter
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Dataset FTP location
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