PXD020774

PXD020774 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems
Description	Prescribed exercise has the potential to ameliorate tissue repair or regeneration and therefore is gaining increasing importance in regenerative rehabilitation. Osteocytes are an appealing type of bone cell to target by regenerative rehabilitation protocols due to their mechanosensitive and secretory nature. Making use of tandem liquid chromatography-mass spectrometry (LC-MSMS) as well as curated bioinformatics, we examined the secretome of mouse and human osteocytic bone cells cultured under cyclic tension delivered by a computer-controlled bioreactor. Quantitative SWATH MS analysis of the secretome secreted by mechanically stimulated cells revealed differential expression of 13 out of 759 secreted factors in mouse and 16 out of 276 in human osteocytic cells. GO enrichment analysis suggested that response to oxidative stress was the most predominant biological process in mouse and cholesterol or lipoprotein-related in human cells. Ossification and bone remodelling and manganese transport were 2 overrepresented process networks common to both mouse and human osteocytic cells
HostingRepository	PRIDE
AnnounceDate	2021-06-08
AnnouncementXML	Submission_2021-06-07_21:36:38.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Boocock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-06 04:43:59	ID requested
⏵ 1	2021-06-07 21:36:39	announced

Dataset with its publication pending

submitter keyword: mouse osteocyte, human osteocyte, SWATH, secretome, tissue regeneration, mechanically stimulated

David John Boocock
contact affiliation	John van Geest Cancer Research Centre, Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
lab head
David Boocock
contact affiliation	Nottingham Trent University
contact email	david.boocock@ntu.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020774
     1. Label: PRIDE project
     2. Name: Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems