PXD020774 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems |
Description | Prescribed exercise has the potential to ameliorate tissue repair or regeneration and therefore is gaining increasing importance in regenerative rehabilitation. Osteocytes are an appealing type of bone cell to target by regenerative rehabilitation protocols due to their mechanosensitive and secretory nature. Making use of tandem liquid chromatography-mass spectrometry (LC-MSMS) as well as curated bioinformatics, we examined the secretome of mouse and human osteocytic bone cells cultured under cyclic tension delivered by a computer-controlled bioreactor. Quantitative SWATH MS analysis of the secretome secreted by mechanically stimulated cells revealed differential expression of 13 out of 759 secreted factors in mouse and 16 out of 276 in human osteocytic cells. GO enrichment analysis suggested that response to oxidative stress was the most predominant biological process in mouse and cholesterol or lipoprotein-related in human cells. Ossification and bone remodelling and manganese transport were 2 overrepresented process networks common to both mouse and human osteocytic cells |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-08 |
AnnouncementXML | Submission_2021-06-07_21:36:38.845.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | David Boocock |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-06 04:43:59 | ID requested | |
⏵ 1 | 2021-06-07 21:36:39 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: mouse osteocyte, human osteocyte, SWATH, secretome, tissue regeneration, mechanically stimulated |
Contact List
David John Boocock |
contact affiliation | John van Geest Cancer Research Centre, Nottingham Trent University |
contact email | david.boocock@ntu.ac.uk |
lab head | |
David Boocock |
contact affiliation | Nottingham Trent University |
contact email | david.boocock@ntu.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020774
- Label: PRIDE project
- Name: Transcriptome and secretome analysis of mechanically stretched osteocytic cells reveals activation of signalling pathways implicated in bone regeneration and DNA-repair systems