PXD020762 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative Proteomic Analysis of MLK Family Kinases in Arabidopsis |
Description | Protein phosphorylation is one of the most prevalent post-translational modifications found in eukaryotic systems and serves as a key molecular mechanism by which protein function is regulated in response to environmental stimuli. The Mut9-Like Kinases (MLKs) are a plant-specific family of Ser/Thr kinases that have been linked to light, circadian, and abiotic stress signaling. Here we use quantitative phosphoproteomics in conjunction with global proteomic analysis to explore the role of the MLKs in daily protein dynamics. In the absence of MLK family kinases,proteins involved in light, circadian, and hormone signaling as well as several chromatin modifying enzymes were found to have altered phosphorylation profiles. Additionally, mlk mutant seedlings were found to have elevated glucosinolate accumulation and increased sensitivity to DNAdamage. Our analysis in combination with previously reported data supports the involvement of MLKs in a diverse set of stress responses and developmental processes, suggesting that the MLKs may serve as key regulators linking environmental inputs to developmental outputs. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-12 |
AnnouncementXML | Submission_2021-03-11_22:24:34.758.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD020762 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dmitri Nusinow |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-06 00:22:52 | ID requested | |
⏵ 1 | 2021-03-11 22:24:35 | announced | |
Publication List
Wilson ME, Tzeng SC, Augustin MM, Meyer M, Jiang X, Choi JH, Rogers JC, Evans BS, Kutchan TM, Nusinow DA, Quantitative Proteomics and Phosphoproteomics Support a Role for Mut9-Like Kinases in Multiple Metabolic and Signaling Pathways in Arabidopsis. Mol Cell Proteomics, 20():100063(2021) [pubmed] |
Keyword List
submitter keyword: Arabidopsis, phosphoproteomic, LC-MS/MS |
Contact List
Dmitri A. Nusinow |
contact affiliation | Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA |
contact email | dnusinow@danforthcenter.org |
lab head | |
Dmitri Nusinow |
contact affiliation | Donald Danforth Plant Science Center |
contact email | dnusinow@danforthcenter.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020762
- Label: PRIDE project
- Name: Quantitative Proteomic Analysis of MLK Family Kinases in Arabidopsis