PXD020762

PXD020762 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Analysis of MLK Family Kinases in Arabidopsis
Description	Protein phosphorylation is one of the most prevalent post-translational modifications found in eukaryotic systems and serves as a key molecular mechanism by which protein function is regulated in response to environmental stimuli. The Mut9-Like Kinases (MLKs) are a plant-specific family of Ser/Thr kinases that have been linked to light, circadian, and abiotic stress signaling. Here we use quantitative phosphoproteomics in conjunction with global proteomic analysis to explore the role of the MLKs in daily protein dynamics. In the absence of MLK family kinases,proteins involved in light, circadian, and hormone signaling as well as several chromatin modifying enzymes were found to have altered phosphorylation profiles. Additionally, mlk mutant seedlings were found to have elevated glucosinolate accumulation and increased sensitivity to DNAdamage. Our analysis in combination with previously reported data supports the involvement of MLKs in a diverse set of stress responses and developmental processes, suggesting that the MLKs may serve as key regulators linking environmental inputs to developmental outputs.
HostingRepository	PRIDE
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-03-11_22:24:34.758.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020762
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dmitri Nusinow
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-06 00:22:52	ID requested
⏵ 1	2021-03-11 22:24:35	announced

Wilson ME, Tzeng SC, Augustin MM, Meyer M, Jiang X, Choi JH, Rogers JC, Evans BS, Kutchan TM, Nusinow DA, Quantitative Proteomics and Phosphoproteomics Support a Role for Mut9-Like Kinases in Multiple Metabolic and Signaling Pathways in Arabidopsis. Mol Cell Proteomics, 20():100063(2021) [pubmed]

submitter keyword: Arabidopsis, phosphoproteomic, LC-MS/MS

Dmitri A. Nusinow
contact affiliation	Donald Danforth Plant Science Center, St. Louis, MO, 63132, USA
contact email	dnusinow@danforthcenter.org
lab head
Dmitri Nusinow
contact affiliation	Donald Danforth Plant Science Center
contact email	dnusinow@danforthcenter.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020762
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Analysis of MLK Family Kinases in Arabidopsis