PXD020759 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative interactomics of Lck-TurboID in living human T cells unveils T cell receptor stimulation-induced proximal Lck interactors |
Description | While Lck has been widely recognized to play a pivotal role in the initiation of the T cell receptor (TCR) signaling pathway, an understanding of the precise regulation of Lck in T cells upon TCR activation remains elusive. Investigation of protein-protein interaction (PPI) using proximity labeling techniques such as TurboID has the potential to provide valuable molecular insights into Lck regulatory networks. By expressing Lck-TurboID in Jurkat T cells, we have uncovered a dynamic, short-range Lck protein interaction network upon 30 minutes of TCR stimulation. In this novel application of TurboID, we detected 27 TCR stimulation-induced Lck-proximal interactors in living T cells, including both bona fide and uncanonical Lck interactors, validating the discovery power of this tool. Our results revealed previously unappreciated Lck PPI which may be associated with cytoskeletal rearrangement, ubiquitination of TCR signaling proteins, activation of the MAPK cascade, coalescence of the LAT signalosome, and formation of the immunological synapse. In this study, we demonstrated for the first time in immune cells and for the kinase Lck that TurboID can be utilized to unveil PPI dynamics in living cells at a time scale consistent with TCR signaling. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-13 |
AnnouncementXML | Submission_2020-11-13_05:42:24.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xien Yu Chua |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-05 23:20:04 | ID requested | |
⏵ 1 | 2020-11-13 05:42:25 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: TurboID, Lck, proximity labeling, interactome, T cell receptor |
Contact List
Arthur, Salomon |
contact affiliation | Department of molecular biology, cell biology and biochemistry, Laboratory of Molecular Medicine, Brown University, United States |
contact email | arthur_salomon@brown.edu |
lab head | |
Xien Yu Chua |
contact affiliation | Brown University |
contact email | xien_yu_chua@brown.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020759
- Label: PRIDE project
- Name: Quantitative interactomics of Lck-TurboID in living human T cells unveils T cell receptor stimulation-induced proximal Lck interactors