PXD020751

PXD020751 is an original dataset announced via ProteomeXchange.

Title	Host-interactor screens of Phytophthora infestans RXLR proteins
Description	Pathogens modulate host cell structure and function by secreting effectors into host tissues. Effectors generally function by associating with host molecules and co-opting or perturbing their activities. This study aimed at identifying the plant proteins associated with the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans in order to identify the host processes targeted by these effectors. We carried out an in planta screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by co-immunoprecipitation (co-IP) and liquid chromatography tandem mass spectrometry (LC-MS/MS). This generated an effector-host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes that are candidate effector-targeted pathways. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of vesicle trafficking machinery. One member of this family, PexRD31, increased the number of GFP-2xFYVE labeled vesicles in N. benthamiana cells. We also noted an accumulation of FYVE vesicles in areas of N. benthamiana leaves colonized by P. infestans indicating that this pathogen may enhance endosomal trafficking. We hope that the interactome dataset we generated will serve as a useful community resource for functional studies of P. infestans effectors.
HostingRepository	PRIDE
AnnounceDate	2020-09-23
AnnouncementXML	Submission_2021-03-10_22:05:21.836.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020751
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jan Sklenar
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100; scientific name: Phytophthora infestans; NCBI TaxID: 4787;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 10:01:28	ID requested
1	2020-09-23 07:06:53	announced
⏵ 2	2021-03-10 22:05:22	announced	2021-03-11: Updated publication reference for PubMed record(s): 33677602.

Petre B, Contreras MP, Bozkurt TO, Schattat MH, Sklenar J, Schornack S, Abd-El-Haliem A, Castells-Graells R, Lozano-Dur, á, n R, Dagdas YF, Menke FLH, Jones AME, Vossen JH, Robatzek S, Kamoun S, Win J, Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. Plant Cell, 33(5):1447-1471(2021) [pubmed]

submitter keyword: Phytophthora infestans, RXLR effector proteins, Nicotiana Benthamiana

Jan Sklenar
contact affiliation	The Sainsbury Laboratory, Proteomics group, Norwich, UK
contact email	jan.sklenar@tsl.ac.uk
lab head
Jan Sklenar
contact affiliation	The Sainsbury Laboratory, Norwich, UK
contact email	jan.sklenar@tsl.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020751

PRIDE project URI

• PRIDE
  1. PXD020751
     1. Label: PRIDE project
     2. Name: Host-interactor screens of Phytophthora infestans RXLR proteins