PXD020748

PXD020748 is an original dataset announced via ProteomeXchange.

Title	Label-free quantification (LFQ) analysis of the AtACINUS interactome
Description	AtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modifications on AtACINUS. A homozygous 35S::AtACINUS-GFP/acinus-2 plant was selected for similar protein expression level to the endogenous AtACINUS protein of wild-type plants using a native α-AtACINUS antibody. We isolated putative AtACINUS interaction partners from young Arabidopsis seedlings using a modified LaG16LaG2 nanobody. Plants expressing TAP-GFP under 35S promoter were used as controls.
HostingRepository	PRIDE
AnnounceDate	2020-12-02
AnnouncementXML	Submission_2020-12-01_23:18:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shouling Xu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 09:59:52	ID requested
⏵ 1	2020-12-01 23:18:34	announced

Dataset with its publication pending

submitter keyword: Plant, seedling, Arabidopsis thaliana, Label-free quantification analysis, LFQ, AtACINUS, ACINUS, co-immunoprecipitation, LC-MS/MS, IP-MS

SHOULING XU
contact affiliation	Carnegie Mass Spectrometry Facility
contact email	sxu@ciw.edu
lab head
Shouling Xu
contact affiliation	Carnegie Institution at Stanford
contact email	sxu@ciw.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020748
     1. Label: PRIDE project
     2. Name: Label-free quantification (LFQ) analysis of the AtACINUS interactome