PXD020745

PXD020745 is an original dataset announced via ProteomeXchange.

Title	compartmentalized protein expression in rat hippocampal neurons undergoing homeostatic plasticity
Description	Synaptic scaling is a form of homeostatic plasticity which allows neurons to adjust their action potential firing rate in response to chronic alterations in neural activity. Synaptic scaling requires profound changes in gene expression, but the relative contribution of local and cell-wide mechanisms is controversial. Here we performed a comprehensive multi-omics characterization of the somatic and process compartments of primary rat hippocampal neurons during synaptic scaling. Thereby, we uncovered highly compartment-specific and correlated changes in the neuronal transcriptome and proteome. Specifically, we identified highly compartment-specific downregulation of crucial regulators of neuronal excitability and excitatory synapse structure. Motif analysis further suggests an important role for trans-acting post-transcriptional regulators, including RNA-binding proteins and microRNAs, in the local regulation of the corresponding mRNAs. Altogether, our study indicates that compartmentalized gene expression changes are widespread in synaptic scaling and might co-exist with neuron-wide mechanisms to allow synaptic computation and homeostasis
HostingRepository	PRIDE
AnnounceDate	2021-07-09
AnnouncementXML	Submission_2021-07-09_04:11:07.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lukas von Ziegler
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 09:41:43	ID requested
⏵ 1	2021-07-09 04:11:08	announced

Dataset with its publication pending

submitter keyword: rat, cellculture, neurons, dendrites, PTX, stimulation, homeostatic plasticity, hippocampus

Gerhard Schratt
contact affiliation	Lab of Systems Neuroscience, Institute for Neuroscience, Department of Health Science and Technology, Swiss Federal Institute of Technology ETH, 8057 Zurich, Switzerland
contact email	gerhard.schratt@hest.ethz.ch
lab head
Lukas von Ziegler
contact affiliation	Swiss Federal Institute of Technology
contact email	lukasvonziegler@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020745
     1. Label: PRIDE project
     2. Name: compartmentalized protein expression in rat hippocampal neurons undergoing homeostatic plasticity