PXD020734

PXD020734 is an original dataset announced via ProteomeXchange.

Title	Peli1-mediated TSC1 ubiquitination site
Description	Metabolic fitness of T cells is crucial for immune responses against infections and tumorigenesis. Both the T cell receptor (TCR) signal and environmental cues contribute to the induction of T cell metabolic reprogramming, but the underlying mechanism is incompletely understood. Here we identified the E3 ubiquitin ligase Peli1 as an important regulator of T cell metabolism and antitumor immunity. Peli1 ablation profoundly promotes tumor rejection, associated with increased tumor-infiltrating CD4 and CD8 T cells. The Peli1-deficient T cells display markedly stronger metabolic activities, particularly glycolysis, than wildtype T cells. Peli1 controls the activation of a metabolic kinase, mTORC1, stimulated by both the TCR signal and growth factors, and this function of Peli1 is mediated through regulation of the mTORC1-inhibitory proteins, TSC1 and TSC2. Peli1 mediates non-degradative ubiquitination of TSC1, thereby promoting TSC1-TSC2 dimerization and TSC2 stabilization. These results establish Peli1 as an important regulator of T cell metabolism and antitumor immunity and suggest a novel mechanism that controls mTORC1 activation.
HostingRepository	PRIDE
AnnounceDate	2020-11-23
AnnouncementXML	Submission_2020-11-22_23:08:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chun-Jung Ko
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-05 06:19:42	ID requested
⏵ 1	2020-11-22 23:08:14	announced

Dataset with its publication pending

submitter keyword: Peli1

TSC1

ubiquitination

Shao-Cong Sun
contact affiliation	Department of Immunology, The University of Texas MD Anderson Cancer Center, 7455 Fannin Street, Box 902, Houston, Texas, USA
contact email	ssun@mdanderson.org
lab head
Chun-Jung Ko
contact affiliation	Department of Immunology, MD Anderson Cancer Center
contact email	CKo@mdanderson.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020734
     1. Label: PRIDE project
     2. Name: Peli1-mediated TSC1 ubiquitination site