PXD020723

PXD020723 is an original dataset announced via ProteomeXchange.

Title	Influenza viral RNA pulldown MS in THP-1 cells
Description	Due to the RNA nature of their genomes, influenza viruses have to utilize many RNA-binding proteins (RBPs) of both viral and host origin, for their replication. To uncover the comprehensive vRNA-host protein interactions, we performed affinity purification coupled with mass spectrometry (AP-MS) analysis of influenza vRNA complexes. The eight vRNA segments of H7N9 were transcribed and individually labeled with biotin in vitro and incubated with IAV virus-infected THP-1 cells, and vRNA complexes were enriched streptavidin magnetic beads and analyzed by mass spectrometry
HostingRepository	PRIDE
AnnounceDate	2021-03-25
AnnouncementXML	Submission_2021-03-24_23:28:08.208.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020723
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhimin Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q TRAP

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-04 04:55:28	ID requested
⏵ 1	2021-03-24 23:28:08	announced

Dataset with its publication pending

submitter keyword: Influenza virus,Viral RNA, RNA pulldown, THP-1 cells, AP-MS

Liu jinhua
contact affiliation	Chair of Department of Preventive Veterinary Medicine College of Veterinary Medicine China Agricultural University (CAU)
contact email	ljh@cau.edu.cn
lab head
Zhimin Jiang
contact affiliation	China Agriculture University
contact email	jiang_zm@cau.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020723

PRIDE project URI

• PRIDE
  1. PXD020723
     1. Label: PRIDE project
     2. Name: Influenza viral RNA pulldown MS in THP-1 cells