PXD020714

PXD020714 is an original dataset announced via ProteomeXchange.

Title	Comparative quantitative LC-MS/MS analysis of 13 amylase/trypsin inhibitors in ancient and modern Triticum species
Description	Amylase/trypsin inhibitors (ATIs) are major wheat allergens and they are also implicated in causing non-celiac gluten sensitivity and worsening other inflammatory conditions. With only few studies on ATI contents in different Triticum species available so far, we developed a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) method based on stable isotope dilution assays to quantitate the 13 most important ATIs in a well-defined sample set of eight cultivars of common wheat and durum wheat (modern species), as well as spelt, emmer and einkorn (ancient species) grown at three locations in Germany, respectively. Only few ATIs with low contents were detected in einkorn. In contrast, spelt had the highest total ATI contents. Emmer and common wheat had similar total ATI contents, with durum wheat having lower contents than common wheat. Due to the lack of correlation, it was not possible to estimate ATI contents based on crude protein contents. The wheat species had a higher influence on ATI contents than the growing location and the heritability of this trait was high. Despite comparatively low intra-species variability, some cultivars were identified that may be promising candidates for breeding for naturally low ATI contents.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-03-12_19:35:34.054.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Sabrina Geisslitz
SpeciesList	scientific name: Triticum; NCBI TaxID: 4564;
ModificationList	Gln->pyro-Glu; Carbamidomethyl; Oxidation; Label:13C(5)15N(1); Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(6)15N(1)
Instrument	TSQ Vantage

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 08:37:03	ID requested
⏵ 1	2021-03-12 19:35:34	announced

Geisslitz S, Longin CFH, Koehler P, Scherf KA, Comparative quantitative LC-MS/MS analysis of 13 amylase/trypsin inhibitors in ancient and modern Triticum species. Sci Rep, 10(1):14570(2020) [pubmed]

submitter keyword: Amylase/trypsin inhibitors

einkorn

emmer

LC-MS/MS

mass spectrometry

non-celiac gluten sensitivity (NCGS)

spelt

stable isotope dilution assay

wheat

Katharina Scherf
contact affiliation	Karlsruhe Institute of Technology
contact email	katharina.scherf@kit.edu
lab head
Sabrina Geisslitz
contact affiliation	Karlsruhe Institute of Technology
contact email	sabrina.geisslitz@kit.edu
dataset submitter

Panorama Public dataset URI