PXD020711

PXD020711 is an original dataset announced via ProteomeXchange.

Title	Gu-Ben-Fang-Xiao decoction ameliorates airway remodeling by regulating the PI3K/AKT signaling pathway mediated by RTN4 in a chronic remission asthma murine model
Description	Airway remodeling is a main pathological feature of asthma. The current therapy for asthma is mainly targeted for reducing inflammation, not particularly for airway remodeling. Herein, developing alternative and more effective therapy to attenuate remodeling is worthy of further study. Gu-Ben-Fang-Xiao Decoction (GBFXD) has been used to treat asthma for decades effectively and safely. In present study, GBFXD significantly regulated the airway inflammation, collagen deposition and the molecules relevant to airway remodeling such as Vimentin, α-SMA, hydroxyproline (HYP), and E-cadherin in chronic remission asthma (CRA) murine model. Subsequently, we found the overlapping differentially expressed proteins (DEPs) between Model/Control and GBFXD/Model mainly belonged to Collagen and Laminin which were extracellular matrix (ECM) proteins by iTRAQ proteomics. In addition, the KEGG analysis showed GBFXD could regulate pathways related to airway remodeling, such as ECM-receptor interaction, Focal adhesion, and PI3K/AKT signaling pathway which were top three pathways of most DEPs (Model/Control and GBFXD/Model) significantly enriched simultaneously. Further validation research showed GBFXD regulated Reticulon-4 (RTN4), thus suppressing the activation of PI3K/AKT pathway to alleviate the ECM proteins deposition. Thus, our findings indicate GBFXD regulate the PI3K/AKT pathway via RTN4 to improve airway remodeling and provide a new insight into the molecular mechanism of GBFXD for treating CRA.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_00:19:48.550.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiongqiong Xing
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 06:31:33	ID requested
⏵ 1	2021-09-09 00:19:50	announced

Xing Q, You Y, Zhao X, Ji J, Yan H, Dong Y, Ren L, Ding Y, Hou S, iTRAQ-Based Proteomics Reveals Gu-Ben-Fang-Xiao Decoction Alleviates Airway Remodeling via Reducing Extracellular Matrix Deposition in a Murine Model of Chronic Remission Asthma. Front Pharmacol, 12():588588(2021) [pubmed]

submitter keyword: chronic remission asthma, Gu-Ben-Fang-Xiao decoction, airway remodeling, RTN4, PI3K/AKT pathway

Xia Zhao
contact affiliation	Affiliated Hospital of Nanjing University of Chinese Medicine
contact email	zhaoxiahy@njucm.edu.cn
lab head
Qiongqiong Xing
contact affiliation	Nanjing University of Chinese Medicine
contact email	1458602752@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020711
     1. Label: PRIDE project
     2. Name: Gu-Ben-Fang-Xiao decoction ameliorates airway remodeling by regulating the PI3K/AKT signaling pathway mediated by RTN4 in a chronic remission asthma murine model