PXD020710

PXD020710 is an original dataset announced via ProteomeXchange.

Title	Multiple drug-induced stress responses inhibit formation of Escherichia coli biofilms
Description	In most ecosystems, bacteria primarily exist as structured surface-associated biofilms that can be highly tolerant to antibiotics and thus represent an important health issue. Here we explored drug repurposing as a strategy to identify new antibiofilm compounds, screening over 1000 compounds from the Prestwick Chemical Library of approved drugs for specific activities that prevent biofilm formation by Escherichia coli. Most growth-inhibiting compounds, which include known antibacterial but also antiviral and other drugs, had also reduced biofilm formation. However, we also identified several drugs that were biofilm-inhibitory at doses where only weak or no effect on planktonic growth could be observed. Activities of the most specific antibiofilm compounds were further characterized using gene expression analysis, proteomics and microscopy. We observed that most of these drugs acted by repressing genes responsible for production of curli, major component of E. coli biofilm matrix. This repression apparently occurred through induction of several different stress responses, including DNA and cell-wall damage, and homeostasis of divalent cations, demonstrating that biofilm formation can be inhibited through a variety of molecular mechanisms. One tested drug, tyloxapol, inhibited biofilm formation independent of curli expression or growth, by suppressing bacterial attachment at the surface.
HostingRepository	PRIDE
AnnounceDate	2020-08-14
AnnouncementXML	Submission_2020-09-11_03:17:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Witold Szymanski
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 06:31:32	ID requested
1	2020-08-14 03:16:42	announced
⏵ 2	2020-09-11 03:17:46	announced	2020-09-11: Updated publication reference for PubMed record(s): 32826218.

Teteneva NA, Mart'yanov SV, Esteban-L, ó, pez M, Kahnt J, Glatter T, Netrusov AI, Plakunov VK, Sourjik V, Multiple Drug-Induced Stress Responses Inhibit Formation of Escherichia coli Biofilms. Appl Environ Microbiol, 86(21):(2020) [pubmed]

submitter keyword: Escherichia coli, LC-MSMS, stress, drug

Victor Sourjik
contact affiliation	Max Planck Institute for Terrestrial Microbiology Karl-von-Frisch Strasse 10, 35043 Marburg, Germany Center for Synthetic Microbiology (SYNMIKRO), Karl-von-Frisch Strasse 10, 35043 Marburg, Germany
contact email	victor.sourjik@synmikro.mpi-marburg.mpg.de
lab head
Witold Szymanski
contact affiliation	Max Planck Institute for Terrestrial MIcrobiology
contact email	witold.szymanski@mpi-marburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD020710

PRIDE project URI

• PRIDE
  1. PXD020710
     1. Label: PRIDE project
     2. Name: Multiple drug-induced stress responses inhibit formation of Escherichia coli biofilms