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PXD020709

PXD020709 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSpatiotemporal Profiling of Cytosolic Signaling Complexes in Living Cells by Highly Selective Proximity Proteomics
DescriptionAPEX2-mediated proximity labeling pioneers in situ capture of spatiotemporal protein complexes in living cells but is limited by its high background. Here, we developed an optimized probe BP5 with much lower labeling noise. Combining the new proximity labeling with label-free quantitative proteomics, we made pair-wise comparison with current version of biotin phenol probe and show the high selectivity of our newly developed probe. Moreover, wesystematically characterized spatiotemporal interactome of a previously less appreciated EGFR signaling core component STS1 at five time points. The side-by-side comparison with affinity purification-mass spectrometry confirmed the comparable performance for exploring EGFR core complexes. This study provided a proof of concept that our new system can be an effective system broadly applied to investigate transient protein-protein interactions.
HostingRepositoryPRIDE
AnnounceDate2020-11-18
AnnouncementXMLSubmission_2020-11-18_03:25:55.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKe Mi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-03 06:31:32ID requested
12020-11-18 03:25:56announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Protein complex, Proximity labeling, Spatiotemporal, Proteomics
Contact List
Ruijun Tian
contact affiliationBiological mass spectrometry and proteomics group, Department of Chemistry High-resolution mass spectrometry platform, Core research facilities Guangdong provincial key laboratory for cell microenvironment and disease research Southern University of Science and Technology (SUSTech) 403, 1st research building, 1088 Xueyuan Rd., Nanshan, Shenzhen
contact emailtianrj@sustech.edu.cn
lab head
Ke Mi
contact affiliationP.h.d student
contact email11930781@mail.sustech.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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