PXD020700 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | 15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome |
Description | AtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modifications on AtACINUS. A homozygous 35S::AtACINUS-GFP/acinus-2 plant was selected for similar protein expression level to the endogenous AtACINUS protein of wild-type plants using a native α-AtACINUS antibody. We isolated putative AtACINUS interaction partners from young Arabidopsis seedlings using the native α-AtACINUS antibody. Plants expressing TAP-GFP under 35S promoter were used as controls. |
HostingRepository | PRIDE |
AnnounceDate | 2020-12-02 |
AnnouncementXML | Submission_2020-12-01_23:16:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shouling Xu |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-03 02:40:21 | ID requested | |
⏵ 1 | 2020-12-01 23:16:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Plant, seedling, Arabidopsis thaliana, 15N stable-isotope-labeling in Arabidopsis(SILIA), AtACINUS, ACINUS, co-immunoprecipitation, LC-MS/MS, IP-MS |
Contact List
SHOULING XU |
contact affiliation | Carnegie Mass Spectrometry Facility |
contact email | sxu@ciw.edu |
lab head | |
Shouling Xu |
contact affiliation | Carnegie Institution at Stanford |
contact email | sxu@ciw.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020700
- Label: PRIDE project
- Name: 15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome