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PXD020700

PXD020700 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title15N Stable-isotope-labeling in Arabidopsis (SILIA) quantitative MS analysis of the AtACINUS interactome
DescriptionAtACINUS protein is involved in regulation of alternative transcription and splicing(AS). Identifying interaction partners and protein complex compositions for AtACINUS can produce valuable information on the mechanisms by which they regulate transcription and AS, as well as post-translational modifications on AtACINUS. A homozygous 35S::AtACINUS-GFP/acinus-2 plant was selected for similar protein expression level to the endogenous AtACINUS protein of wild-type plants using a native α-AtACINUS antibody. We isolated putative AtACINUS interaction partners from young Arabidopsis seedlings using the native α-AtACINUS antibody. Plants expressing TAP-GFP under 35S promoter were used as controls.
HostingRepositoryPRIDE
AnnounceDate2020-12-02
AnnouncementXMLSubmission_2020-12-01_23:16:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShouling Xu
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-03 02:40:21ID requested
12020-12-01 23:16:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plant, seedling, Arabidopsis thaliana, 15N stable-isotope-labeling in Arabidopsis(SILIA), AtACINUS, ACINUS, co-immunoprecipitation, LC-MS/MS, IP-MS
Contact List
SHOULING XU
contact affiliationCarnegie Mass Spectrometry Facility
contact emailsxu@ciw.edu
lab head
Shouling Xu
contact affiliationCarnegie Institution at Stanford
contact emailsxu@ciw.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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