PXD020689

PXD020689 is an original dataset announced via ProteomeXchange.

Title	Affinity Purification Trigger with mTPP
Description	We have applied a BASIL-like approach to increase the signal of low abundance peptides to the study of protein complexes. To accomplish this, we incorporated an affinity purified cleavage and polyadenylation factor (CPF) complex as a “trigger” into our mTPP workflow at a trigger sample to lowest heat-treated mTPP sample ratio of ~1:8 and ~1:50. We saw a significant increase in the abundance of CPF complex members, including those that were not readily identified without the trigger. Importantly, addition of a trigger into our mTPP workflow does not appear to have a significant impact on the melt temperature (Tm) calculation of proteins detected both with and without the trigger. Use of purified protein complex trigger channels in TPP studies, and potentially other global proteomics applications, will improve the ability to perform proteomic analysis of low abundance protein complexes. As many biologically-relevant, as well as disease-relevant, protein complexes are of relatively low abundance in the cell, improving the detection of such proteins in proteomics experiments would be beneficial to increasing our under-standing of the critical cellular processes these complexes play roles in.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_01:45:37.756.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020689
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sarah Peck Justice
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 02:38:48	ID requested
⏵ 1	2021-09-09 01:45:38	announced

Peck Justice SA, McCracken NA, Victorino JF, Qi GD, Wijeratne AB, Mosley AL, Boosting Detection of Low-Abundance Proteins in Thermal Proteome Profiling Experiments by Addition of an Isobaric Trigger Channel to TMT Multiplexes. Anal Chem, 93(18):7000-7010(2021) [pubmed]

submitter keyword: TPP, AP-MS, LC-MS/MS

Amber L. Mosley
contact affiliation	Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN
contact email	almosley@iu.edu
lab head
Sarah Peck Justice
contact affiliation	Indiana University School of Medicine
contact email	sapeck@iu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020689
     1. Label: PRIDE project
     2. Name: Affinity Purification Trigger with mTPP