PXD020688

PXD020688 is an original dataset announced via ProteomeXchange.

Title	Profiling of Y1 cells treated with FGF-2 reveals parallels with oncogene-induced senescence
Description	Paradoxically, oncogenes that drive cell cycle progression may also trigger pathways leading to senescence, thereby inhibiting the growth of tumorigenic cells. Along these lines, Y1 cells, which carry an amplification of Ras, become senescent after treatment with the mitogen FGF-2. To understand how FGF-2 promotes senescence, we profiled the epigenome, transcriptome, proteome, and phospho-proteome of Y1 cells stimulated with FGF-2. FGF-2 caused delayed acetylation of histone H4 and higher levels of H3K27me3. Sequencing analysis revealed decreased expression of cell cycle-related genes with concomitant loss of H3K27ac. In contrast, FGF-2 promoted the expression of p21, various cytokines, and MAPK-related genes. Nuclear envelope proteins, particularly lamin B1, displayed increased phosphorylation in response to FGF-2. Proteome analysis suggested alterations in cellular metabolism, as evident by modulated expression of enzymes involved in purine biosynthesis, tRNA aminoacylation, and the TCA cycle. Altogether, the response of Y1 cells to FGF-2 is consistent with oncogene-induced senescence. We propose that Y1 cells enter senescence due to deficient cyclin expression and high levels of p21, which may stem from DNA damage or TGFb signaling.
HostingRepository	PRIDE
AnnounceDate	2021-06-16
AnnouncementXML	Submission_2021-06-16_07:54:23.598.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020688
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peder Lund
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-03 02:38:44	ID requested
⏵ 1	2021-06-16 07:54:24	announced

Dataset with its publication pending

submitter keyword: FGF-2, senescence, phosphorylation, cell cycle

Benjamin A. Garcia
contact affiliation	Epigenetics Institute, Dept. of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA
contact email	bgarci@pennmedicine.upenn.edu
lab head
Peder Lund
contact affiliation	University of Pennsylvania
contact email	pederjlund@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020688
     1. Label: PRIDE project
     2. Name: Profiling of Y1 cells treated with FGF-2 reveals parallels with oncogene-induced senescence