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PXD020679

PXD020679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic signatures of corals from thermodynamic reefs
DescriptionIn this work, I took an iTRAQ approach to profile the proteomes of 12 coral samples. Specifically, corals were collected from an upwelling site in the Luzon Strait ("Houbihu") and a non-upwelling "control" site in the nearby Taiwan Strait ("Houwan") and exposed to either highly variable (upwelling-simulating) or stable temperatures in the laboratory for seven days prior to sacrificing for protein analysis (the "Seriatopora hystrix variable temperature study" [SHVTS]). In this submission, I have uploaded the manuscript describing the experiment (Mayfield et al. 2012 J Exp Biol), the proofs of the manuscript describing the proteomic data (Mayfield in press Microorganisms), two RAW files (SHVTS-A & SHVTS-B for iTRAQ batches A & B, respectively), two MZML files (SHVTS-A & SHVTS-B), two MZID files (SHVTS-A & SHVTS-B), and a tab-delineated file that describes which samples were labelled with which iTRAQ tag ("Mayfield online supplemental data file"). I have also uploaded the supplemental material that accompanies the Mayfield (in press) article since that is what readers interested in the specifics of the proteomic analysis pipeline should consult. The peak data were queried against a conceptually translated S. hystrix-Cladocopium dinoflagellate transcriptome that has been uploaded as a fasta file. Please note that all proteomic data represent a mix of multiple organisms (namely the anthozoan host and the dinoflagellate endosymbionts that inhabit their gastrodermal cells). All data analysis was conducted with Proteome Discoverer (Thermo-Fisher Scientific).
HostingRepositoryMassIVE
AnnounceDate2020-08-01
AnnouncementXMLSubmission_2020-08-01_09:20:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAnderson B Mayfield
SpeciesList scientific name: Cladocopium sp. clade C; NCBI TaxID: 293275; scientific name: Seriatopora hystrix; common name: bird's nest coral; NCBI TaxID: 51070;
ModificationListiTRAQ8plex
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-31 10:38:12ID requested
12020-08-01 09:20:38announced
Publication List
Mayfield AB. Proteomic Signatures of Corals from Thermodynamic Reefs. Mayfield AB (2020) Proteomic Signatures of Corals from Thermodynamic Reefs. Microorganisms (in press).
Keyword List
submitter keyword: coral reefs, dinoflagellates, proteomics, iTRAQ, symbiosis, thermal biology
Contact List
Anderson Mayfield
contact affiliationUniversity of Miami
contact emailabm64@miami.edu
lab head
Anderson B Mayfield
contact affiliationNOAA
contact emailanderson.mayfield@noaa.gov
dataset submitter
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