PXD020670

PXD020670 is an original dataset announced via ProteomeXchange.

Title	Glycometabolic regulation of the biogenesis of small extracellular vesicles
Description	The biogenesis of small extracellular vesicles (sEVs) is regulated by multiple molecular machineries, generating considerably heterogeneous vesicle populations, i.e., exosomes and non-exosomal vesicles, with distinct cargo molecules. However, the role of carbohydrate metabolism in generating such vesicle heterogeneity remains largely elusive. Here we discovered that 2-deoxyglucose (2-DG), a well-known glycolysis inhibitor, suppressed the secretion of non-exosomal vesicles by impairing asparagine-linked glycosylation (N-glycosylation) in mouse melanoma cells. In the present study, to gain deeper insight into the molecular signatures of non-exosomal vesicles, we performed label-free quantitative proteomics of sEVs released from cells that were treated with or without 2-DG or NGI-1.
HostingRepository	PRIDE
AnnounceDate	2020-10-14
AnnouncementXML	Submission_2020-10-22_00:00:03.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020670
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Takehiro Suzuki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-31 01:36:16	ID requested
1	2020-10-13 23:34:47	announced
⏵ 2	2020-10-22 00:00:04	announced	2020-10-22: Updated publication reference for PubMed record(s): 33053347.

Harada Y, Nakajima K, Suzuki T, Fukushige T, Kondo K, Seino J, Ohkawa Y, Suzuki T, Inoue H, Kanekura T, Dohmae N, Taniguchi N, Maruyama I, Glycometabolic Regulation of the Biogenesis of Small Extracellular Vesicles. Cell Rep, 33(2):108261(2020) [pubmed]

submitter keyword: exosome

Naoshi Dohmae
contact affiliation	Biomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact email	dohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliation	RIKEN Center for Sustainable Resource Science
contact email	takehirosuzuki@riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD020670

PRIDE project URI

• PRIDE
  1. PXD020670
     1. Label: PRIDE project
     2. Name: Glycometabolic regulation of the biogenesis of small extracellular vesicles