PXD020666

PXD020666 is an original dataset announced via ProteomeXchange.

Title	Protein interactome profiling of ER alpha by RIME assay from the human hepatocellular cancer HepG2 stable cells
Description	Estrogeh insensitivity syndrome (EIS) arises from rare mutations in ERα resulting in the inability of estrogen to exert its biological effects. Due to the rarity, mutations in ESR1 gene and the underlying molecular mechanisms of EIS have not been thoroughly studied. We used RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins) analysis to compare the interactions for ER alpha between the WT ER alpha and Q375H clinical mutant in HepG2 stable cells.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:42:59.226.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020666
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yin Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-31 01:35:42	ID requested
⏵ 1	2021-09-09 02:43:00	announced

Li Y, Coons LA, Houtman R, Carlson KE, Martin TA, Mayne CG, Melchers D, Jefferson TB, Ramsey JT, Katzenellenbogen JA, Korach KS, associated with estrogen insensitivity affects the coupling between ligand binding and coactivator recruitment. Sci Signal, 13(650):(2020) [pubmed]

submitter keyword: ER alpha mutation, protein interactome, cell model

Yin Li
contact affiliation	Reproductive & Developmental Biology Laboratory, NIEHS, NIH, USA.
contact email	liy4@niehs.nih.gov
lab head
Yin Li
contact affiliation	NIEHS/NIH
contact email	liy4@niehs.nih.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020666
     1. Label: PRIDE project
     2. Name: Protein interactome profiling of ER alpha by RIME assay from the human hepatocellular cancer HepG2 stable cells