PXD020665

PXD020665 is an original dataset announced via ProteomeXchange.

Title	Identification of new proteins related with cisplatin resistance in S. cerevisiae
Description	The aim of this study is to select a cisplatin resistant Saccharomyces cerevisiae strain as a model organism to look for new molecular markers of cisplatin resistance and the identification of mechanisms/interactions involved. A cisplatin resistant S. cerevisiae strain was obtained after continuous exposure to the drug during 80 days. Then, total protein extraction, purification and identification were carried out, in wild type (wt) and resistant strains, by tandem mass spectrometry using a "nano HPLC-ESI-MS/MS" ion trap system. The increase in emPAI (resistant vs wt strains) was calculated to study the increase in protein expression. "Genemania" software (http://www.Genemania.org/) was used to compare the effects, functions and interactions of proteins. The selected cisplatin resistant strain showed 2.5 times more resistance than the wt strain (for the ID50 value) and 2.78 times more resistant for the ID90 value. The long-term exposure to cisplatin induced resistance in S. cerevisiae, obtaining an increased expression of QCR2, QCR1, ALDH4, ATPB, ATPA, SCW10, HSP26, ATPG, and PCKA proteins. The overexpression of the above-mentioned proteins suggests that they could be involved in cisplatin resistance. The resistance acquisition process is complex and involves the activation of multiple mechanisms that interact together.
HostingRepository	PRIDE
AnnounceDate	2021-05-28
AnnouncementXML	Submission_2021-05-27_21:45:31.736.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Ruiz-Gomez
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	reduction of disulfide crosslink in cystine to two cysteines; alkylated residue; iodoacetamide derivatized amino-terminal residue
Instrument	Mascot; ProteinScape; DB source UniProt; DB source NCBI; Bruker Daltonics amaZon series

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-31 01:35:29	ID requested
⏵ 1	2021-05-27 21:45:32	announced

Burgos-Molina AM, Mercado-S, á, enz S, C, á, rdenas C, L, ó, pez-D, í, az B, Sendra-Portero F, Ruiz-G, ó, mez MJ, Identification of new proteins related with cisplatin resistance in Saccharomyces cerevisiae. Appl Microbiol Biotechnol, 105(5):1965-1977(2021) [pubmed]

submitter keyword: Yeast, Saccharomyces cerevisiae, proteomic, cisplatin, resistance

Miguel J. Ruiz-Gómez
contact affiliation	Universidad de Málaga, Facultad de Medicina, Departamento de Radiología y Medicina Física, Málaga, España
contact email	mjrg@uma.es
lab head
Miguel Ruiz-Gomez
contact affiliation	University of Malaga
contact email	mjrg@uma.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020665
     1. Label: PRIDE project
     2. Name: Identification of new proteins related with cisplatin resistance in S. cerevisiae