PXD020664

PXD020664 is an original dataset announced via ProteomeXchange.

Title	Living the sweet life: how Liquorilactobacillus hordei TMW 1.1822 changes its behavior in the presence of sucrose in comparison to glucose
Description	Liquorilactobacillus (L.) hordei (formerly Lactobacillus hordei) is one of the dominating lactic acid bacteria within the water kefir consortium, being highly adapted to survive in this environment, while producing high molecular weight dextrans from sucrose. In this work, we extensively studied the physiological response of L. hordei TMW 1.1822 to sucrose compared to glucose, applying label-free, quantitative proteomics of cell lysates and exoproteomes. This revealed the differential expression of 53 proteins within cellular proteomes, mostly associated with carbohydrate uptake and metabolism. Supported by growth experiments, this suggests L. hordei TMW 1.1822 to favor fructose over other sugars. The dextransucrase was expressed irrespective of the present carbon source, while it was significantly more released in the presence of sucrose (log2FC = 3.09), being among the most abundant proteins within exoproteomes of sucrose-treated cells. Still, L. hordei TMW 1.1822 expressed other sucrose active enzymes, predictively competing with the dextransucrase reaction. While osmolysis appeared to be unlikely, sucrose led to an increased release of a multitude of cytoplasmic proteins, suggesting that biofilm formation in L. hordei is not only composed of a polysaccharide matrix, but also of proteinaceous nature. Therefore, our study highlights the intrinsic adaptation of water kefir-borne L. hordei to sucrose-rich habitats and provides fundamental knowledge for the use as starter culture in plant-based food fermentations with in situ dextran formation.
HostingRepository	PRIDE
AnnounceDate	2021-01-07
AnnouncementXML	Submission_2021-01-07_00:15:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christina Ludwig
SpeciesList	scientific name: Lactobacillus hordei; NCBI TaxID: 468911;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-30 22:34:31	ID requested
⏵ 1	2021-01-07 00:15:46	announced

Bechtner J, Ludwig C, Kiening M, Jakob F, Vogel RF, TMW 1.1822 Changes Its Behavior in the Presence of Sucrose in Comparison to Glucose. Foods, 9(9):(2020) [pubmed]

submitter keyword: Liquorilactobacillus hordei, Lactobacillus hordei, dextransucrase, proteomics, exoproteome, water kefir, sucrose, metabolism

Christina Ludwig
contact affiliation	Bavarian Center for Biomolecular Mass Spectrometry (BayBioMS) Technische Universität München (TUM) Gregor-Mendel-Straße 4 85354 Freising GERMANY
contact email	tina.ludwig@tum.de
lab head
Christina Ludwig
contact affiliation	TU Munich
contact email	tina.ludwig@tum.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020664
     1. Label: PRIDE project
     2. Name: Living the sweet life: how Liquorilactobacillus hordei TMW 1.1822 changes its behavior in the presence of sucrose in comparison to glucose