PXD020661

PXD020661 is an original dataset announced via ProteomeXchange.

Title	An insight into Exosomal Cargo Isolated from three tissue specific Mesenchymal Stem Cells: Implication in Translational Research
Description	Adult Mesenchymal stem cells (MSCs) derived exosomes have recently gained importance as a cell-free therapy for several chronic and inflammatory diseases. It is believed that these exosomes contain several biologically active molecules (i.e. miRNAs, proteins) which help to cure the diseases. The majority of published reports to date that have investigated the secretome of MSCs have done so using canonical expansion of cell culture conditions. However, the microenvironment experience by MSC post administration into animal models and patients is strikingly different. Thus instead of using secretome, Exosomes purified from adipose tissue (AD), Wharton Jelly (WJ) and Bone marrow BM) are widely studied. However, the content of the exosomes may differ based on their sources from where they are originated. It remains unclear, however, which types of proteins are packaged into exosomes compared with the cells from which they are derived. To explore these exosomes as a cell-free therapy for specific diseases, it is therefore, important to get comprehensive knowledge of the bioactive molecules present in the exosomes. In this study, we have purified the exosomes from three different sources (AD, WJ, and BM) and using RNA-Seq approach, we have categorized the common and different microRNAs present in the exosomes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:36.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arup Banerjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	amidated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-30 22:23:37	ID requested
1	2023-04-26 05:24:46	announced
⏵ 2	2023-11-14 08:51:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: stem cells,exosome

ARUP BANERJEE
contact affiliation	REGIONAL CENTRE FOR bIOTECHNOLOGY, FARIDABAD, INDIA
contact email	arup@rcb.res.in
lab head
Arup Banerjee
contact affiliation	Regional Centre for Biotechnology, Faridabad, India
contact email	arup@rcb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020661
     1. Label: PRIDE project
     2. Name: An insight into Exosomal Cargo Isolated from three tissue specific Mesenchymal Stem Cells: Implication in Translational Research