PXD020657

PXD020657 is an original dataset announced via ProteomeXchange.

Title	FluoroTRAQ: Quantitative Analysis of Protein S-nitrosylation through Fluorous Solid-phase Extraction Combining with iTRAQ by Mass Spectrometry
Description	S-nitrosylation is an important post-translational modification which occurs on cysteine in organisms. S-nitrosylation regu-lates signal transduction in many cell processes and aberrant S-nitrosylated (SNO-) proteins are closely related to cardiovas-cular and neurological diseases. However, quantitative analysis of the S-nitrosylation at the proteome level remains challeng-ing due to the low abundance of the modification. Herein, we developed a novel approach called FluoroTRAQ, which com-bined the fluorous solid-phase extraction of SNO-peptides and the iTRAQ labeling for the quantitative analysis of SNO-proteome with high sensitivity and specificity. The new analytical strategy is successfully applied to monitor of the dynamic changes of SNO-proteome in cells. A quantitative dataset of SNO-proteome in human umbilical vein endothelial cell (HUVEC) after in vivo S-nitrosoglutathione (GSNO) stimulation was revealed. Among them, several new SNO-proteins in-cluding DHX9, GOT2 et al. are reported and validated by biotin switch assay.
HostingRepository	iProX
AnnounceDate	2020-07-30
AnnouncementXML	Submission_2021-03-25_20:51:39.217.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoli Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-nitrosyl-L-cysteine
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-30 19:03:58	ID requested
1	2020-07-30 19:05:42	announced
⏵ 2	2021-03-25 20:51:39	announced	2021-03-26: Update publication information.

Zhang C, Xu Y, Wang G, Fang C, Bao H, Zhang Y, Lu H, FluoroTRAQ: Quantitative Analysis of Protein S-Nitrosylation through Fluorous Solid-Phase Extraction Combining with iTRAQ by Mass Spectrometry. Anal Chem, 92(23):15317-15322(2020) [pubmed]

submitter keyword: S-nitrosylation

Haojie Lu
contact affiliation	Fudan University
contact email	luhaojie@fudan.edu.cn
lab head
Guoli Wang
contact affiliation	Fudan University
contact email	19111510006@fudan.edu.cn
dataset submitter

iProX dataset URI