PXD020657
PXD020657 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | FluoroTRAQ: Quantitative Analysis of Protein S-nitrosylation through Fluorous Solid-phase Extraction Combining with iTRAQ by Mass Spectrometry |
Description | S-nitrosylation is an important post-translational modification which occurs on cysteine in organisms. S-nitrosylation regu-lates signal transduction in many cell processes and aberrant S-nitrosylated (SNO-) proteins are closely related to cardiovas-cular and neurological diseases. However, quantitative analysis of the S-nitrosylation at the proteome level remains challeng-ing due to the low abundance of the modification. Herein, we developed a novel approach called FluoroTRAQ, which com-bined the fluorous solid-phase extraction of SNO-peptides and the iTRAQ labeling for the quantitative analysis of SNO-proteome with high sensitivity and specificity. The new analytical strategy is successfully applied to monitor of the dynamic changes of SNO-proteome in cells. A quantitative dataset of SNO-proteome in human umbilical vein endothelial cell (HUVEC) after in vivo S-nitrosoglutathione (GSNO) stimulation was revealed. Among them, several new SNO-proteins in-cluding DHX9, GOT2 et al. are reported and validated by biotin switch assay. |
HostingRepository | iProX |
AnnounceDate | 2020-07-30 |
AnnouncementXML | Submission_2021-03-25_20:51:39.217.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guoli Wang |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | S-nitrosyl-L-cysteine |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-07-30 19:03:58 | ID requested | |
1 | 2020-07-30 19:05:42 | announced | |
⏵ 2 | 2021-03-25 20:51:39 | announced | 2021-03-26: Update publication information. |
Publication List
Zhang C, Xu Y, Wang G, Fang C, Bao H, Zhang Y, Lu H, FluoroTRAQ: Quantitative Analysis of Protein S-Nitrosylation through Fluorous Solid-Phase Extraction Combining with iTRAQ by Mass Spectrometry. Anal Chem, 92(23):15317-15322(2020) [pubmed] |
Keyword List
submitter keyword: S-nitrosylation |
Contact List
Haojie Lu | |
---|---|
contact affiliation | Fudan University |
contact email | luhaojie@fudan.edu.cn |
lab head | |
Guoli Wang | |
contact affiliation | Fudan University |
contact email | 19111510006@fudan.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |