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PXD020657

PXD020657 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFluoroTRAQ: Quantitative Analysis of Protein S-nitrosylation through Fluorous Solid-phase Extraction Combining with iTRAQ by Mass Spectrometry
DescriptionS-nitrosylation is an important post-translational modification which occurs on cysteine in organisms. S-nitrosylation regu-lates signal transduction in many cell processes and aberrant S-nitrosylated (SNO-) proteins are closely related to cardiovas-cular and neurological diseases. However, quantitative analysis of the S-nitrosylation at the proteome level remains challeng-ing due to the low abundance of the modification. Herein, we developed a novel approach called FluoroTRAQ, which com-bined the fluorous solid-phase extraction of SNO-peptides and the iTRAQ labeling for the quantitative analysis of SNO-proteome with high sensitivity and specificity. The new analytical strategy is successfully applied to monitor of the dynamic changes of SNO-proteome in cells. A quantitative dataset of SNO-proteome in human umbilical vein endothelial cell (HUVEC) after in vivo S-nitrosoglutathione (GSNO) stimulation was revealed. Among them, several new SNO-proteins in-cluding DHX9, GOT2 et al. are reported and validated by biotin switch assay.
HostingRepositoryiProX
AnnounceDate2020-07-30
AnnouncementXMLSubmission_2020-07-30_19:05:41.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuoli Wang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListS-nitrosyl-L-cysteine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-30 19:03:58ID requested
12020-07-30 19:05:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: S-nitrosylation
Contact List
Haojie Lu
contact affiliationFudan University
contact emailluhaojie@fudan.edu.cn
lab head
Guoli Wang
contact affiliationFudan University
contact email19111510006@fudan.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI