PXD020656

PXD020656 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of mouse liver and skeletal muscle
Description	The liver and skeletal muscle were collected from 10-week-old wild-type and ob/ob mice fasted for 16 hours. Tissues were frozen immediately and lysed with 0.5 mL of a solution containing 50 mM Tris-HCl pH8.8, 2%SDS, and 7 M urea, and then subjected to ultrasonic treatment with a Bioruptor (Digaenode). The samples were diluted with an equal volume of water and centrifuged at 15,000 g for 15 min at 4°C to remove insoluble fraction. The protein concentration of the lysates was determined with the bicinchoninic acid assay (Thermo Fisher Scientific) and adjusted to 1 mg/mL. Cysteine residues were blocked by incubation of the samples with 2 mM tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher Scientific) for 30 min at 37 °C followed by alkylation with 10 mM 2-iodoacetamide for 30 min at room temperature. The proteins (200 µg) were precipitated with acetone for 3 h at −30 °C and the resulting pellet was dispersed in 50 mM Triethylammonium bicarbonate by ultrasonic treatment (three times for 30 s with intervals of 30 s) with a Bioruptor (Diagenode). The protein suspension was subjected to digestion with lysyl endopeptidase (Wako) for 16 h at 37 °C. Resulting peptides were centrifuged at 15,000 g for 15 min at 4°C and subjected to C18-StageTip purification prior to MS analysis
HostingRepository	jPOST
AnnounceDate	2021-03-03
AnnouncementXML	Submission_2022-09-18_03:28:57.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Atsushi Hatano
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-30 19:00:02	ID requested
1	2021-03-02 17:08:10	announced
2	2021-12-20 20:21:38	announced	2021-12-21: Updated PubMed.
⏵ 3	2022-09-18 03:28:58	announced	2022-09-18: Updated FTP location.

Egami R, Kokaji T, Hatano A, Yugi K, Eto M, Morita K, Ohno S, Fujii M, Hironaka KI, Uematsu S, Terakawa A, Bai Y, Pan Y, Tsuchiya T, Ozaki H, Inoue H, Uda S, Kubota H, Suzuki Y, Matsumoto M, Nakayama KI, Hirayama A, Soga T, Kuroda S, Trans-omic analysis reveals obesity-associated dysregulation of inter-organ metabolic cycles between the liver and skeletal muscle. iScience, 24(3):102217(2021) [pubmed]

submitter keyword: mouse, liver, skeletal muscle

Shinya Kuroda
lab head
Atsushi Hatano
contact affiliation	Department of Omics and Systems Biology, Niigata University
dataset submitter

jPOST dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000927/