The invasion and egress are the two key steps in T. gondii lytic cycle that are vital to the propagation of its infection, and the phosphorylation is believed to play important roles in these processes. In this study, we performed phosphoproteomic analysis of these two dynamic processes in the lytic cycle of T. gondii and identified the phosphoproteins with phosphorylation level significantly changed or not at these two infection stages of T. gondii.