PXD020642

PXD020642 is an original dataset announced via ProteomeXchange.

Title	H2S regulation the metabolism of cucumber in response to salt-stress through transcriptome and proteome analysis
Description	In a previous study, we found that H2S alleviates salinity stress in cucumber by maintaining the Na+/K+ balance and by regulating H2S metabolism and the oxidative stress response. However, little is known about the molecular mechanisms behind H2S-regulated salt-stress tolerance in cucumber. Here, an integrated transcriptomic and proteomic analysis based on RNA-seq and 2-DE was used to investigate the global mechanism underlying H2S-regulated salt-stress tolerance. In total, 11 761 differentially expressed genes (DEGs) and 61 differentially expressed proteins (DEPs) were identified. Analysis of the pathways associated with the DEGs showed that salt stress enriched expression of genes in primary and energy metabolism, such as photosynthesis, carbon metabolism and biosynthesis of amino acids. Application of H2S significantly decreased these DEGs but enriched DEGs related to plant-pathogen interaction, sulfur-containing metabolism, cell defense and signal transduction pathways. Notably, changes related to sulfur-containing metabolism and cell defense were also observed through proteome analysis, such as Cysteine synthase 1, Glutathione S-transferase U25-like, Protein disulfide-isomerase and Peroxidase 2. We present the first global analysis of the mechanism underlying H2S regulation of salt-stress tolerance in cucumber through tracking changes in the expression of specific proteins and genes.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_02:40:12.038.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinglong Jiang
SpeciesList	scientific name: Cucumis sativus; NCBI TaxID: 3659;
ModificationList	monohydroxylated residue
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-29 22:04:39	ID requested
⏵ 1	2021-09-09 02:40:12	announced

Jiang J, Ren X, Li L, Hou R, Sun W, Jiao C, Yang N, Dong Y, S Regulation of Metabolism in Cucumber in Response to Salt-Stress Through Transcriptome and Proteome Analysis. Front Plant Sci, 11():1283(2020) [pubmed]

submitter keyword: Cucumis sativus L., Transcriptomic, Proteomic, Hydrogen sulfide, Salt stress

Jinglong Jiang
contact affiliation	School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China
contact email	jiangjinglong511@163.com
lab head
Jinglong Jiang
contact affiliation	Shaanxi University of Technology
contact email	jiangjinglong511@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020642
     1. Label: PRIDE project
     2. Name: H2S regulation the metabolism of cucumber in response to salt-stress through transcriptome and proteome analysis