PXD020640

PXD020640 is an original dataset announced via ProteomeXchange.

Title	mice lung TMT labeling proteome
Description	Acinetobacter baumannii is an important pathogen of nosocomial infection worldwide, which can primarily cause pneumonia, bloodstream infection, and urinary tract infection. The increasing drug resistance rate of A. baumannii and the slow development of new antibacterial drugs brought great challenges for clinical treatment. Host immunity is crucial to the defense of A. baumannii infection, and understanding the mechanisms of immune response can facilitate the development of new therapeutic strategies. To obtain the system-level changes of host proteome in immune response, we used TMT labeling quantitative proteomics to compare the proteome changes of lungs from A. baumannii infected mice with control mice six hours after infection. A total of 6218 proteins were identified in which 6172 could be quantified. With threshold p < 0.05 and fold change > 1.2, we found 120 differentially expressed proteins. Bioinformatics analysis showed that differentially expressed proteins after infection were associated with receptor recognition, NADPH oxidase (NOX) activation and antimicrobial peptides. These differentially expressed proteins were involved in the pathways including leukocyte transendothelial migration, phagocyte, neutrophil degranulation, and antimicrobial peptides. In conclusion, our study showed proteome changes in mouse lung tissue due to A. baumannii infection and suggested the important roles of NOX, neutrophils, and antimicrobial peptides in host response. Our results provide a potential list of proteins candidates for the further study of host-bacteria interaction in A. baumannii infection.
HostingRepository	PRIDE
AnnounceDate	2021-03-16
AnnouncementXML	Submission_2021-03-15_21:09:01.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-29 07:33:21	ID requested
⏵ 1	2021-03-15 21:09:01	announced

Dataset with its publication pending

submitter keyword: Acinetobacter baumannii, lung infection, host response, TMT-labeling proteomics, NADPH oxidase (NOX)

Jing Zhang
contact affiliation	Fudan University, Huashan Hospital
contact email	zhangj61@fudan.edu.cn
lab head
Xin Li
contact affiliation	Huashan Hospital, Fudan University
contact email	lxxj6311@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020640
     1. Label: PRIDE project
     2. Name: mice lung TMT labeling proteome