PXD020627

PXD020627 is an original dataset announced via ProteomeXchange.

Title	Barley root proteome and metabolome in response to cytokinin
Description	Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes in plants. Its potential for biotechnology and development of high-yield and more resilient plants has been recognized, but the molecular mechanisms behind its action are far from understood. In this report, the roots of barley seedling were explored as a new tool to reveal as yet unknown cytokinin-responsive proteins. Significant differences were reproducibly observed for 176 proteins, and at least some of the revealed cytokinin-responsive pathways were confirmed in metabolome analysis, including alterations in phenylpropanoid pathway, amino acid biosynthesis or ROS metabolism. Bioinformatics analysis indicated a significant overlap between cytokinin response and response to abiotic stress. This was confirmed by comparing proteome and metabolome profiles in response to drought, salinity or a period of temperature stress. The results illustrate complex abiotic stress response in the early development of model crop plant and confirm an extensive crosstalk between plant hormone cytokinin and response to temperature stimuli, water availability or salinity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:13:08.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Cerny
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue; acetylated residue; deamidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-28 23:21:06	ID requested
1	2020-10-05 22:48:56	announced
2	2020-12-01 08:42:54	announced	2020-12-01: Updated publication reference for PubMed record(s): 33250914.
⏵ 3	2024-10-22 05:13:14	announced	2024-10-22: Updated project metadata.

Berka M, Luklov, á M, Dufkov, á H, Berkov, á V, Nov, á, k J, Saiz-Fern, á, ndez I, Rashotte AM, Brzobohat, ý B, Č, ern, ý M, Barley Root Proteome and Metabolome in Response to Cytokinin and Abiotic Stimuli. Front Plant Sci, 11():590337(2020) [pubmed]

10.3389/fpls.2020.590337;

submitter keyword: abiotic stress,Hordeum vulgare, ROS, Zeatin, proteome

Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
lab head
Martin Cerny
contact affiliation	Mendel University in Brno
contact email	martincerny83@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD020627

PRIDE project URI

• PRIDE
  1. PXD020627
     1. Label: PRIDE project
     2. Name: Barley root proteome and metabolome in response to cytokinin