PXD020619

PXD020619 is an original dataset announced via ProteomeXchange.

Title	Another layer of complexity in Staphylococcus aureus methionine biosynthesis control: Unusual RNase III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay
Description	In Staphylococcus aureus, de novo methionine biosynthesis is regulated by a unique hierarchical pathway involving stringent-response controlled CodY repression in combination with a T-box riboswitch and RNA decay. The T-box riboswitch residing in the 5' untranslated region (met leader RNA) of the S. aureus metICFE-mdh operon controls downstream gene transcription upon interaction with uncharged methionyl-tRNA. met leader and metICFE-mdh (m)RNAs undergo RNase-mediated degradation in a process whose molecular details are poorly understood. Here, we determined the secondary structure of the met leader RNA and found the element to harbor, beyond other conserved T-box riboswitch structural features, a terminator helix which is a target for RNase III endoribonucleolytic cleavage. As the terminator is a thermodynamically highly stable structure, it also forms posttranscriptionally in met leader/ metICFE-mdh read-through transcripts. Cleavage by RNase III releases the met leader from metICFE-mdh mRNA and initiates RNase J-mediated degradation of the mRNA from the 5'-end. Of note, metICFE-mdh mRNA stability varies over the length of the transcript with a longer lifespan towards the 3'-end. Corresponding variations in protein levels led us to hypothesize that coordinated RNA decay represents another level in the hierarchical methionine biosynthesis control network to adjust methionine biosynthesis enzyme amounts to current metabolic requirements.
HostingRepository	PRIDE
AnnounceDate	2021-08-10
AnnouncementXML	Submission_2021-08-10_06:54:31.289.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Staphylococcus aureus (strain Newman); NCBI TaxID: 426430;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-28 17:34:18	ID requested
1	2021-08-10 04:04:38	announced
⏵ 2	2021-08-10 06:54:32	announced	2021-08-10: Updated project metadata.

Wencker FDR, Marincola G, Schoenfelder SMK, Maa, ß S, Becher D, Ziebuhr W, III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay. Nucleic Acids Res, 49(4):2192-2212(2021) [pubmed]

10.1093/NAR/GKAA1277;

submitter keyword: methionine biosynthesis, Staphylococcus aureus, RNA decay,T-box riboswitch

Dörte Becher
contact affiliation	Institute of Microbiology, University of Greifswald, Greifswald,Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020619
     1. Label: PRIDE project
     2. Name: Another layer of complexity in Staphylococcus aureus methionine biosynthesis control: Unusual RNase III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay