PXD020601

PXD020601 is an original dataset announced via ProteomeXchange.

Title	Serum proteomics in COVID-19 patients: Altered coagulation and complement status as a function of IL-6 level
Description	Over 5 million people around the world have tested positive for the beta coronavirus SARS-CoV- 2 as of May 29, 2020, a third of which in the United States alone. These infections are associated with the development of a disease known as COVID-19, which is characterized by several symptoms, including persistent dry cough, shortness of breath, chills, muscle pain, headache, loss of taste or smell, and gastrointestinal distress. COVID-19 has been characterized by elevated mortality (over 100 thousand people have already died in the US alone), mostly due to thromboinflammatory complications that impair lung perfusion and systemic oxygenation in the most severe cases. While the levels of pro-inflammatory cytokines such as interleukin-6 (IL-6) have been associated with the severity of the disease, little is known about the impact of IL-6 levels on the proteome of COVID-19 patients. The present study provides the first proteomics analysis of sera from COVID-19 patients, stratified by circulating levels of IL-6, and correlated to markers of inflammation and renal function. As a function of IL-6 levels, we identified significant dysregulation in serum levels of various coagulation factors, accompanied by increased levels of anti-fibrinolytic components, including several serine protease inhibitors (SERPINs). These were accompanied by up-regulation of the complement cascade and antimicrobial enzymes, especially in subjects with the highest levels of IL- 6, which is consistent with an exacerbation of the acute phase response in these subjects. Although our results are observational, they highlight a clear increase in the levels of inhibitory components of the fibrinolytic cascade in severe COVID-19 disease, providing potential clues related to the etiology of coagulopathic complications in COVID-19 and paving the way for potential therapeutic interventions, such as the use of pro-fibrinolytic agents.
HostingRepository	PRIDE
AnnounceDate	2020-07-29
AnnouncementXML	Submission_2020-07-28_23:09:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Hill
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Bruker Daltonics flex series

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-28 01:32:05	ID requested
⏵ 1	2020-07-28 23:09:55	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2

serum

disease severity

clot

inflammation

Kirk Hansen
contact affiliation	Biochemistry and Molecular Genetics
contact email	Kirk.Hansen@cuanschutz.edu
lab head
Ryan Hill
contact affiliation	University of Colorado Anschutz Medical Campus
contact email	Ryan.Hill@ucdenver.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020601
     1. Label: PRIDE project
     2. Name: Serum proteomics in COVID-19 patients: Altered coagulation and complement status as a function of IL-6 level