PXD020589

PXD020589 is an original dataset announced via ProteomeXchange.

Title	An HPF1/PARP1-based chemical biology strategy for exploring ADP-ribosylation
Description	Strategies for installing authentic ADP-ribosylation (ADPr) at desired positions are fundamental for creating the tools needed to explore this elusive PTM in essential cellular processes. Here we describe a phospho-guided chemoenzymatic approach based on the Ser-ADPr writer complex for rapid, scalable preparation of a panel of pure, precisely-modified peptides. Integrating this streamlined methodology with phage display technology, we have developed the first site-specific as well as broad-specificity antibodies to mono-ADPr. These recombinant antibodies have been selected and characterized using multiple ADP-ribosylated peptides and tested by immunoblotting and immunofluorescence for their ability to detect physiological ADPr events. By enabling mono-ADPr proteomics and poly-to-mono comparisons at the modification site level, we have revealed the prevalence of mono-ADPr upon DNA damage and illustrated its dependence on PARG and ARH3. These and future tools created on our versatile chemical biology/recombinant antibody platform have broad potential to elucidate ADPr signaling pathways in health and disease.
HostingRepository	PRIDE
AnnounceDate	2020-11-17
AnnouncementXML	Submission_2020-11-17_08:48:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edoardo José Longarini
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	adenosine diphosphoribosyl (ADP-ribosyl) modified residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-27 02:35:32	ID requested
⏵ 1	2020-11-17 08:48:06	announced

Bonfiglio JJ, Leidecker O, Dauben H, Longarini EJ, Colby T, San Segundo-Acosta P, Perez KA, Matic I, An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation. Cell, 183(4):1086-1102.e23(2020) [pubmed]

submitter keyword: ADP-ribosylation, PARP1, LC-MS, Fusion

Ivan Matic
contact affiliation	Max Planck Insitute for Biology of Ageing
contact email	imatic@age.mpg.de
lab head
Edoardo José Longarini
contact affiliation	Max-Planck Insitute for Biology of Ageing
contact email	elongarini@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD020589

PRIDE project URI

• PRIDE
  1. PXD020589
     1. Label: PRIDE project
     2. Name: An HPF1/PARP1-based chemical biology strategy for exploring ADP-ribosylation