PXD020584

PXD020584 is an original dataset announced via ProteomeXchange.

Title	Formation of a protein corona on the surface of extracellular vesicles in blood plasma
Description	In this study we tested whether a protein corona is formed around extracellular vesicles (EVs) in biofluids. We isolated nascent EVs of THP1 cells and platelets, and incubated them in EV-depleted platelet-free plasma from healthy subjects and patients with rheumatoid arthritis. EVs were subjected to differential centrifugation, size exclusion chromatography, or density gradient ultracentrifugation followed by mass spectrometry. Plasma protein-coated EVs had a higher density compared to nascent ones and carried numerous newly associated proteins. Interactions between plasma proteins and EVs were confirmed by immune electron microscopy and flow cytometry. Based on the results of this study and data published by others, we identified 9 shared corona proteins (ApoA1, ApoB, ApoC3, ApoE, CO3, CO4B, FIBA, IgHG2 and IgHG4) equally present in association with EVs, viruses and artificial nanoparticles in blood plasma. Since ALBU was also present in association with our nascent EVs, we could not include it in the list. We found induction of protein aggregation by centrifugation of our EV-depleted plasma samples at 12,500 g and the aggregate proteins showed high overlap with the EV corona. However, in contrast to aggregates which had no effect, coated EVs induced an increased expression of TNFa, IL-6 and CD83 of human monocyte-derived dendritic cells. In conclusion, our data shed new light on the commonly reported plasma protein “contamination” of EV preparations.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:08.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lilla Turiák
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-27 00:55:46	ID requested
1	2022-02-15 13:28:09	announced
⏵ 2	2024-10-22 05:32:16	announced	2024-10-22: Updated project metadata.

10.1002/jev2.12140;

T, ó, th E Á, Turi, á, k L, Visnovitz T, Cser, é, p C, M, á, zl, ó A, S, ó, dar BW, F, ö, rs, ö, nits AI, Pet, ő, v, á, ri G, Sebesty, é, n A, Koml, ó, si Z, Drahos L, Kittel Á, Nagy G, B, á, csi A, D, é, nes Á, Gho YS, Szab, ó, -Taylor K É, Buz, á, s EI, Formation of a protein corona on the surface of extracellular vesicles in blood plasma. J Extracell Vesicles, 10(11):e12140(2021) [pubmed]

submitter keyword: protein corona

extracellular vesicles

blood plasma

rheumatoid arthritis

mass spectrometry

aggregation

Edit Buzás
contact affiliation	Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary MTA-SE Immune-Proteogenomics Extracellular Vesicle Research Group, Budapest, Hungary HCEMM-SE Extracellular Vesicles Research Group, Budapest, Hungary
contact email	edit.buzas@gmail.com
lab head
Lilla Turiák
contact affiliation	RCNS HAS, MS Proteomics Research Group
contact email	liliat7@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD020584

PRIDE project URI

• PRIDE
  1. PXD020584
     1. Label: PRIDE project
     2. Name: Formation of a protein corona on the surface of extracellular vesicles in blood plasma