PXD020578
PXD020578 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Enrichment-Free Identification of Native Definitive (EnFIND) O-glycoproteome |
| Description | The detection of O-glycosylation at the proteome level has long been a challenging task and a roadblock for O-linked protein glycosylation research. We report an Enrichment-Free Identification of Native Definitive (EnFIND) O-glycoproteome using Trapped Ion Mobility Spectrometry coupled to TOF Mass Spectrometry (TIMS-TOF MS) for direct analysis of protein O-glycosylation in native samples with minimum sample requirement. This approach enabled separation of O-glycopeptide isomers, resolution of O-glycosites and O-glycoform, reduction of sample complexity, and increased sensitivity, thus greatly enhancing analysis of the O-glycoproteome of cell lysates, human serum and exosomes. In addition, we found that antibodies in human serum are highly O-glycosylated on variable, especially hypervariable regions and constant regions, which significantly increases antibody diversity. This method was used to successfully identify characteristic O-glycosylation features of autoimmune diseases. |
| HostingRepository | iProX |
| AnnounceDate | 2020-07-27 |
| AnnouncementXML | Submission_2020-10-20_21:26:37.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shuaixin Gao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | O-glycosyl-L-threonine; O-glycosyl-L-serine |
| Instrument | time-of-flight |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-07-27 00:04:31 | ID requested | |
| ⏵ 1 | 2020-10-20 21:26:37 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: O-glycoproteome,enrichment-free, nanoLC-TIMS TOF MS, human autoimmune diseases |
Contact List
| Catherine CL Wong | |
|---|---|
| contact affiliation | Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University |
| contact email | catherine_wong@bjmu.edu.cn |
| lab head | |
| Shuaixin Gao | |
| contact affiliation | Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University |
| contact email | gaoshuaixin@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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