PXD020560

PXD020560 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	An organometallic gold(I) bis-N-heterocyclic carbene complex with a multimodal mode of action in ovarian cancer cells
Description	The organometallic gold(I) bis-N-heterocyclic carbene complex [Au(9-methylcaffeine-8-ylidene)2]+ (AuTMX2) was previously shown to selectively and potently stabilise telomeric DNA G-quadruplex (G4) structures. This study sheds light onto the molecular reactivity and mode of action of AuTMX2 in the cellular context by using mass spectrometry-based shotgun proteomics in A2780 ovarian cancer cells. In contrast to other metal-based anticancer agents, this organogold compound showed a minor tendency for forming coordinative bonds and is expected to exert its drug effects mainly by non-covalent interactions. Global protein expression changes of treated cancer cells revealed a multi-modal mode of action of AuTMX2 by alterations in the nucleolus, telomeres, actin stress-fibers and stress-responses, which were further supported by pharmacological assays, fluorescence microscopy and cellular accumulation experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:12:28.065.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020560
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christopher Gerner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-24 05:47:40	ID requested
1	2020-09-17 03:41:31	announced
⏵ 2	2024-10-22 05:12:30	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD020560;
Meier-Menches SM, Neuditschko B, Zappe K, Schaier M, Gerner MC, Schmetterer KG, Del Favero G, Bonsignore R, Cichna-Markl M, Koellensperger G, Casini A, Gerner C, An Organometallic Gold(I) Bis-N-Heterocyclic Carbene Complex with Multimodal Activity in Ovarian Cancer Cells. Chemistry, 26(67):15528-15537(2020) [pubmed]
10.1002/chem.202003495;

10.6019/PXD020560;

Meier-Menches SM, Neuditschko B, Zappe K, Schaier M, Gerner MC, Schmetterer KG, Del Favero G, Bonsignore R, Cichna-Markl M, Koellensperger G, Casini A, Gerner C, An Organometallic Gold(I) Bis-N-Heterocyclic Carbene Complex with Multimodal Activity in Ovarian Cancer Cells. Chemistry, 26(67):15528-15537(2020) [pubmed]

10.1002/chem.202003495;

Keyword List

submitter keyword: gold complexes • N-heterocyclic carbenes • G-quadruplexes • telomeres • proteomics • cancer

submitter keyword: gold complexes • N-heterocyclic carbenes • G-quadruplexes • telomeres • proteomics • cancer

Contact List

Christopher Gerner
contact affiliation	Department of Analytical Chemistry, University of Vienna, Austria
contact email	christopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliation	University of Vienna
contact email	christopher.gerner@univie.ac.at
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020560
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020560

PRIDE project URI

Repository Record List

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