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PXD020560

PXD020560 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn organometallic gold(I) bis-N-heterocyclic carbene complex with a multimodal mode of action in ovarian cancer cells
DescriptionThe organometallic gold(I) bis-N-heterocyclic carbene complex [Au(9-methylcaffeine-8-ylidene)2]+ (AuTMX2) was previously shown to selectively and potently stabilise telomeric DNA G-quadruplex (G4) structures. This study sheds light onto the molecular reactivity and mode of action of AuTMX2 in the cellular context by using mass spectrometry-based shotgun proteomics in A2780 ovarian cancer cells. In contrast to other metal-based anticancer agents, this organogold compound showed a minor tendency for forming coordinative bonds and is expected to exert its drug effects mainly by non-covalent interactions. Global protein expression changes of treated cancer cells revealed a multi-modal mode of action of AuTMX2 by alterations in the nucleolus, telomeres, actin stress-fibers and stress-responses, which were further supported by pharmacological assays, fluorescence microscopy and cellular accumulation experiments.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:12:28.065.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD020560
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterChristopher Gerner
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-24 05:47:40ID requested
12020-09-17 03:41:31announced
22024-10-22 05:12:30announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD020560;
Meier-Menches SM, Neuditschko B, Zappe K, Schaier M, Gerner MC, Schmetterer KG, Del Favero G, Bonsignore R, Cichna-Markl M, Koellensperger G, Casini A, Gerner C, An Organometallic Gold(I) Bis-N-Heterocyclic Carbene Complex with Multimodal Activity in Ovarian Cancer Cells. Chemistry, 26(67):15528-15537(2020) [pubmed]
10.1002/chem.202003495;
Keyword List
submitter keyword: gold complexes • N-heterocyclic carbenes • G-quadruplexes • telomeres • proteomics • cancer
Contact List
Christopher Gerner
contact affiliationDepartment of Analytical Chemistry, University of Vienna, Austria
contact emailchristopher.gerner@univie.ac.at
lab head
Christopher Gerner
contact affiliationUniversity of Vienna
contact emailchristopher.gerner@univie.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
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