PXD020559

PXD020559 is an original dataset announced via ProteomeXchange.

Title	ATP13A3 is a major component of the enigmatic mammalian polyamine transport system
Description	Polyamines, including putrescine (PUT), spermidine (SPD) and spermine (SPM), are abundant polycations supporting various cellular functions. Their cellular content is tightly controlled by biosynthesis, degradation and uptake or export via the polyamine transport system (PTS). However, the mammalian PTS remains poorly characterized. Mutated Chinese hamster ovary cells, CHO-MG, are frequently used to study the PTS owing to their phenotype of reduced polyamine uptake and resistance to methylglyoxal bis-(guanylhydrazone) (MGBG), a toxic polyamine biosynthesis inhibitor. Yet, the underlying genetic defect remains enigmatic. We recently proved that the P5B-type ATPase, ATP13A2, is a polyamine transporter and here, we analyzed whether the P5B-type ATPases are deficient in CHO-MG. Label-free shogun proteomics found that ATP13A3 expression is reduced in CHO-MG. Mutations in the ATP13A3 gene were found to critically disturb the protein sequence. Interestingly, depleting ATP13A3 in the wild-type CHO cells induced a CHO-MG phenotype, whereas its complementation in CHO-MG rescued the phenotype. Together, we demonstrate that defective ATP13A3 contributes to the CHO-MG phenotype, designating ATP13A3 as a new member of the mammalian PTS that may partially overlap in function with ATP13A2.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:16:24.388.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD020559
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rupert Mayer
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-24 03:47:10	ID requested
1	2020-12-08 23:42:19	announced
⏵ 2	2024-10-22 05:16:25	announced	2024-10-22: Updated project metadata.

10.1074/jbc.ra120.013908;

Hamouda NN, Van den Haute C, Vanhoutte R, Sannerud R, Azfar M, Mayer R, Cort, é, s Calabuig Á, Swinnen JV, Agostinis P, Baekelandt V, Annaert W, Impens F, Verhelst SHL, Eggermont J, Martin S, Vangheluwe P, ATP13A3 is a major component of the enigmatic mammalian polyamine transport system. J Biol Chem, 296():100182(2021) [pubmed]

10.6019/PXD020559;

submitter keyword: Polyamines, Putrescine, Q Exactive HF,ATP13A3, P5B-ATPase, CHO cells, Polyamine transport system

Peter Vangheluwe
contact affiliation	Laboratory of Cellular Transport Systems, Dpt. of Cellular and Molecular Medicine, KU Leuven, Leuven, Belgium
contact email	peter.vangheluwe@kuleuven.be
lab head
Rupert Mayer
contact affiliation	VIB-UGent Center for Medical Biotechnology, VIB, 9052 Ghent, Belgium.
contact email	rupert.mayer@vib-ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020559
     1. Label: PRIDE project
     2. Name: ATP13A3 is a major component of the enigmatic mammalian polyamine transport system