PXD020542

PXD020542 is an original dataset announced via ProteomeXchange.

Title	Proximal proteome and ubiquitination landscape of E3 Ubiquitin Ligase Mindbomb 1
Description	To characterize the proximal proteome of Mindbomb 1 (MIB1) during adenovirus (AdV) infection ?we performed proximity labeling. MIB1 KO cell lines generated in the Hap1 cell line were stably reconstituted with either WT MIB1-APEX2, the MIB1 ubiquitination-defective mutant C985S-APEX2 or APEX2-mKate. Cell lines were infected with ?replication-competent (rep-comp) AdV5-GFP followed by pulse-labeling with H202 at 1.25 ?hours post infection (hpi), streptavidin isolation of biotinylated proteins and LC-MS/MS analysis of the proximal proteomes. To determine possible u?biquit?ination targets of MIB1 during AdV infection, Tandem-Ubiquitin-Binding-Entities (TUBEs) were used to isolate endogenous ubiquitinated proteins. MIB1 KO Hap1 cells, and KO cells reconstituted with either WT MIB1 or the ubiquitination-dead mutant C985S MIB1 were infected with rep-comp AdV5-GFP and collected at 1 hpi. Lysates were incubated with TUBE Dynabeads, and the isolated proteins were submitted for LC-MS/M
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:28:32.523.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soren Heissel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-23 17:46:56	ID requested
1	2021-09-09 03:02:06	announced
⏵ 2	2024-10-22 05:28:33	announced	2024-10-22: Updated project metadata.

Sarbanes SL, Blomen VA, Lam E, Heissel S, Luna JM, Brummelkamp TR, Falck-Pedersen E, Hoffmann HH, Rice CM, E3 ubiquitin ligase Mindbomb 1 facilitates nuclear delivery of adenovirus genomes. Proc Natl Acad Sci U S A, 118(1):(2021) [pubmed]

10.1073/pnas.2015794118;

submitter keyword: APEX2,Adenovirus,Mindbomb 1, LFQ, TUBEs

Charles M. Rice
contact affiliation	Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY 10065, USA
contact email	ricec@rockefeller.edu
lab head
Soren Heissel
contact affiliation	Research Support Specialist
contact email	sheissel@rockefeller.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD020542

PRIDE project URI

• PRIDE
  1. PXD020542
     1. Label: PRIDE project
     2. Name: Proximal proteome and ubiquitination landscape of E3 Ubiquitin Ligase Mindbomb 1