PXD020537

PXD020537 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Label free mass spectrometry-based quantification of linker histone H1 variants in clinical samples
Description	Epigenetic aberrations have been recognized as important contributors to cancer onset and development, and increasing evidence suggests that linker histone H1 variants may serve as biomarkers useful for patient stratification, as well as play an important role as drivers in cancer. Although traditionally histone H1 levels have been studied using antibody-based methods and RNA expression, these approaches suffer from limitations. Mass-spectrometry (MS)-based proteomics represents the ideal tool to accurately quantify relative changes in protein abundance within complex samples. In this study, we used a label-free quantification approach to simultaneously analyze all somatic histone H1 variants in clinical samples, and verified its applicability to laser microdissected tissue areas containing as low as 1000 cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:03.957.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberta Noberini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-23 17:44:40	ID requested
1	2021-03-16 04:53:11	announced
2	2023-05-14 14:03:48	announced	2023-05-14: Updated project metadata.
⏵ 3	2024-10-22 05:20:04	announced	2024-10-22: Updated project metadata.

Publication List

Noberini R, Morales Torres C, Savoia EO, Brandini S, Jodice MG, Bertalot G, Bonizzi G, Capra M, Diaferia G, Scaffidi P, Bonaldi T, Label-Free Mass Spectrometry-Based Quantification of Linker Histone H1 Variants in Clinical Samples. Int J Mol Sci, 21(19):(2020) [pubmed]
10.3390/ijms21197330;

Noberini R, Morales Torres C, Savoia EO, Brandini S, Jodice MG, Bertalot G, Bonizzi G, Capra M, Diaferia G, Scaffidi P, Bonaldi T, Label-Free Mass Spectrometry-Based Quantification of Linker Histone H1 Variants in Clinical Samples. Int J Mol Sci, 21(19):(2020) [pubmed]

10.3390/ijms21197330;

Keyword List

ProteomeXchange project tag: EPIC-XS
submitter keyword: proteomics, mass spectrometry, label-free quantification,epigenetics, linker histone H1, laser micro-dissection

ProteomeXchange project tag: EPIC-XS

submitter keyword: proteomics, mass spectrometry, label-free quantification,epigenetics, linker histone H1, laser micro-dissection

Contact List

Tiziana Bonaldi
contact affiliation	European Institute of Oncology, Milan
contact email	tiziana.bonaldi@ieo.it
lab head
Roberta Noberini
contact affiliation	Istituto Europeo di Oncologia
contact email	roberta.noberini@ieo.it
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020537
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD020537

PRIDE project URI

Repository Record List

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