PXD020527

PXD020527 is an original dataset announced via ProteomeXchange.

Title	C. acnes CNS catheter infection elicits modest early inflammatory changes but long term alterations in the CSF proteome
Description	Shunt infections lead to grave neurologic morbidity for patients especially when there is a delay in diagnosis. C. acnes is the third most common cause of cerebrospinal fluid (CSF) shunt infection and is underdiagnosed due to the difficulty in culturing this fastidious, slow growing pathogen. Currently the gold standard for diagnosis of CSF shunt infections is microbiologic culture, however, in the case of C. acnes diagnostic cultures may be falsely negative. Therefore, new diagnostic methods are needed. To investigate potential CSF biomarkers of C. acnes CSF shunt infection we adapted a previously published rat model of CSF shunt infection to C. acnes. We found elevated levels of IL-1β, IL-6, CCL2 and IL-10 in the CSF and brain tissues of animals implanted with C. acnes infected catheters compared to sterile controls at day 1 post-infection. We found modest increases in neutrophils in the CSF and to a greater extend the brain tissue of animals with C. acnes infection which mirrors the clinical findings in C. acnes shunt infection. Mass spectrometry revealed that the CSF proteome is altered during C. acnes shunt infection and changes over the course of infection with an acute phase and pathogen neutralization response at day 1 post-infection to a more biosynthetic and metabolic response at day 28 post-infection relating to healing. Collectively, these results demonstrate that it is possible to distinguish C. acnes infection from sterile post-operative inflammation and CSF proteins could be useful in a diagnostic strategy for this pathogen that is difficult to diagnose.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:11.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ishwor Thapa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-23 03:03:15	ID requested
1	2022-02-16 03:54:45	announced
⏵ 2	2024-10-22 05:32:19	announced	2024-10-22: Updated project metadata.

10.1128/iai.00531-20;

Beaver M, Lagundzin D, Thapa I, Lee J, Ali H, Kielian T, Skar GL, Central Nervous System Catheter Infection Induces Long-Term Changes in the Cerebrospinal Fluid Proteome. Infect Immun, 89(4):(2021) [pubmed]

submitter keyword: cytokine, inflammation, CSF, proteome, biomarker, C. acnes,Shunt Infection

Gwenn L. Skar
contact affiliation	University of Nebraska Medical Center 982162 Nebraska Medical Center Omaha, NE 68198-2162
contact email	gskar@unmc.edu
lab head
Ishwor Thapa
contact affiliation	University of Nebraska at Omaha
contact email	ithapa@unomaha.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020527
     1. Label: PRIDE project
     2. Name: C. acnes CNS catheter infection elicits modest early inflammatory changes but long term alterations in the CSF proteome